| Mercy College of Health Sciences | 501(c)(3) | 1946 | $23.2 million | $109.3 million | 2025-06 |
| Institute for Missionary Evangalism | 501(c)(3) | 1990 | $1.3 million | $802,210 | 2025-12 |
| Christ-Life Ministries Inc | 501(c)(3) | 2001 | $500,376 | $1.4 million | 2025-12 |
| Holy Spirit Unity Church in America | 501(c)(3) | 2017 | $495,107 | $1.4 million | 2023-12 |
| Interfaith Alliance of Iowa | 501(c)(3) | 1996 | $454,408 | $170,917 | 2025-12 |
| Polk County Jail Ministries Inc | 501(c)(3) | 1995 | $213,244 | $409,872 | 2025-12 |
| Emmanuel Chin Baptist Church | 501(c)(3) | 1955 | $175,934 | $62,688 | 2022-12 |
| Wat Lao Buddhavath of Iowa | 501(c)(3) | 2005 | $171,208 | $146,178 | 2025-12 |
| Hong Duc Sakya Monastery | 501(c)(3) | 2014 | $149,700 | $494,038 | 2025-12 |
| Campus Fellowship Inc | 501(c)(3) | 2022 | $122,178 | $50,768 | 2025-06 |
| Midwest Christian Ministries | 501(c)(3) | 2011 | $118,113 | $1.2 million | 2024-12 |
| Faith at Work Inc | 501(c)(3) | 2014 | $79,085 | $10,856 | 2024-12 |
| Greek Orthodox Church of St George Foundation | 501(c)(3) | 2013 | $78,788 | $1.1 million | 2025-12 |
| The Bridge-A Vision for Shared Downtown Ministry | 501(c)(3) | 2012 | $78,064 | $112,430 | 2025-12 |
| CPC of The-Wmm-Des Moines Ia-One Inc | 501(c)(3) | 2015 | $73,727 | $112,441 | 2022-12 |
| Willowbrook Bible Camp | 501(c)(3) | 1977 | $64,705 | $59,097 | 2025-11 |
| Jason Fath Ministries | 501(c)(3) | 2015 | $63,166 | $14,297 | 2025-12 |
| Emmaus Way | 501(c)(3) | 1998 | $51,887 | $10,392 | 2025-12 |
| Hindu Cultural and Educational Center | 501(c)(3) | 2016 | $47,306 | $871,440 | 2024-12 |
| Gods Outreach Deliverance Int | 501(c)(3) | 2017 | $15,514 | $1 | 2020-12 |
| African Missions Center Incorporation World Missions for | 501(c)(3) | 2012 | $0 | $0 | 2025-12 |
| Alexander Christian Foundation | 501(c)(3) | 1993 | $0 | $0 | 2025-12 |
| Amazing Grace Ministries and Outreach Church | 501(c)(3) | 1999 | | | |
| Asokarama Arakkha Buddhist Society | 501(c)(3) | 2013 | | | |
| Bethany Reformed Church of the Reformed Church in America | 501(c)(3) | 2023 | | | |
| Bond of Unity Embassy Center | 501(c)(3) | 2018 | $0 | $0 | 2025-12 |
| Broken Vessels Quaker Ministries | 501(c)(3) | 2016 | $0 | $0 | 2025-06 |
| Calming Waters Inc | 501(c)(3) | 2025 | $0 | $0 | 2024-12 |
| Cambodian Buddhist Society Inc | 501(c)(3) | 2010 | | | |
| Centro Cristiano Palabra Viva | 501(c)(3) | 1964 | | | |
| Christ Our Righteousness Worship Center Inc | 501(c)(3) | 2019 | | | |
| Community Christian Family Church | 501(c)(3) | 2022 | | | |
| Community Church of Christ | 501(c)(3) | 2002 | | | |
| Corinthian Baptist Church Inc | 501(c)(3) | 1985 | | | |
| Cottage Grove Church of Des Moines Inc | 501(c)(3) | 2023 | | | |
| Crossroads Community Church | 501(c)(3) | 2006 | | | |
| Crusaders for Christ Church Inc | 501(c)(3) | 2007 | | | |
| Des Moines Vietnamese Buddhist Association | 501(c)(3) | 2000 | | | |
| Des Moines Zen Center | 501(c)(3) | 2003 | | | |
| Destiny Christian Church | 501(c)(3) | 2007 | | | |
| Disaster Relief Missions Inc | 501(c)(3) | 2011 | $0 | $0 | 2024-12 |
| Eleven Street Church of Christ | 501(c)(3) | 2001 | | | |
| Elim Christian Fellowship of Des Moines Iowa Incorporated | 501(c)(3) | 2003 | | | |
| Empowered To Restore Ministries Intl | 501(c)(3) | 2024 | | | |
| Evangelistic Missionary Church in Christ | 501(c)(3) | 2013 | | | |
| Faith Missionary Baptist Church | 501(c)(3) | 2012 | | | |
| Gods Covenant Deliverance Ministries International | 501(c)(3) | 2026 | | | |
| Gods House of Power Cogic Inc NFP | 501(c)(3) | 2018 | | | |
| Grace United Methodist Foundation of Des Moines | 501(c)(3) | 2012 | | | |
| Greater Life Missionary Baptist Church Inc | 501(c)(3) | 2025 | | | |
| Grow Up Ministries Church | 501(c)(3) | 2015 | | | |
| Heart Studio | 501(c)(3) | 2022 | $0 | $0 | 2025-12 |
| Hebron International Ministries | 501(c)(3) | 2024 | | | |
| House of God International Ministries | 501(c)(3) | 2007 | | | |
| Iglesia Avivamiento Revival Church | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Iglesia El Shaddai | 501(c)(3) | 2016 | | | |
| Iglesia Evangelica Apostoles Y Profetas 2 20 Arbol de Vida | 501(c)(3) | 2023 | | | |
| Iglesia Pentecostes Alfa Omega | 501(c)(3) | 2009 | | | |
| Immersed Community Church | 501(c)(3) | 2001 | $0 | $0 | 2021-12 |
| Independent Latvian Evangelical Church in Iowa | 501(c)(3) | 2004 | | | |
| Inste Global Bible College | 501(c)(3) | 2025 | | | |
| International Encouragers | 501(c)(3) | 2024 | $0 | $0 | 2024-12 |
| Iowa Atheists and Freethinkers | 501(c)(3) | 2009 | $0 | $0 | 2026-06 |
| Iowa Des Moines Karen Baptist Church | 501(c)(3) | 2015 | | | |
| Iowa Mission Church | 501(c)(3) | 2024 | | | |
| Iowa Region of the Jesus Christ Apostolic Churches Inc | 501(c)(3) | 2002 | | | |
| Islamic and Educational Center Ezan of Greater Des Moines | 501(c)(3) | 2009 | | | |
| Jesus Is the Way Ministries | 501(c)(3) | 2016 | | | |
| Kenney Linhart Ministries Inc | 501(c)(3) | 2000 | | | |
| Khmer Krom Association of Iowa Inc | 501(c)(3) | 2016 | | | |
| L S M Church Inc | 501(c)(3) | 2026 | | | |
| Legacy Development Fund | 501(c)(3) | 2023 | | | |
| Life Change Family Church Inc | 501(c)(3) | 2017 | | | |
| Lighthouse Ministries | 501(c)(3) | 2000 | | | |
| Living Sacrifice Ministries | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| Liz Knott Memorial Mission Foundation | 501(c)(3) | 2023 | $0 | $0 | 2023-12 |
| Lubavitch of Iowa Inc | 501(c)(3) | 1996 | | | 2024-12 |
| Mayim Rabim | 501(c)(3) | 2025 | | | |
| Mid-Iowa Health Foundation | 501(c)(3) | 1986 | | $20.2 million | 2025-12 |
| Mineral Ridge Gospel Outreach | 501(c)(3) | 2006 | | | |
| Ministerio Fuente de Agua Viva Inc | 501(c)(3) | 2009 | | | |
| Mizo Church of Iowa Inc | 501(c)(3) | 2018 | | | |
| Mount Carmel Christian Fellowship | 501(c)(3) | 2007 | | | |
| Mt Hebron Missionary Baptist Church of Des Moines Iowa | 501(c)(3) | 2012 | | | |
| Mt Olive Missionary Baptist Church of Des Moines Ia Inc | 501(c)(3) | 2005 | | | |
| Muslim Community Organization | 501(c)(3) | 2009 | $0 | $0 | 2025-12 |
| New Beginning Christ Apostolic Temp Le | 501(c)(3) | 2026 | | | |
| New Generation Ministries Internati Onal Inc | 501(c)(3) | 2026 | | | |
| Phothisomphan Meditation Monastery | 501(c)(3) | 2015 | | | |
| Providence Reformed Church | 501(c)(3) | 2015 | | | |
| Revival Center Outreach Ministries | 501(c)(3) | 2022 | | | |
| Romans 13 8 Foundation Inc | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| Sophia Evangelical Church | 501(c)(3) | 2016 | | | |
| St Johns Full Gospel Baptist Church | 501(c)(3) | 1999 | | | |
| St Mary Coptic Orthodox Church | 501(c)(3) | 1998 | | | |
| St Paul African Methodist Episcopal Church | 501(c)(3) | 2000 | | | |
| Storehouse Ministries Inc | 501(c)(3) | 2008 | | | |
| Sweet Home Deliverance Church | 501(c)(3) | 2011 | | | |
| Tawba Islamic Center | 501(c)(3) | 2018 | | | |
| The Iowa United Methodist Foundation | 501(c)(3) | 2015 | | | |
| Thugging To Loving | 501(c)(3) | 2024 | $0 | $0 | 2024-12 |
| True Vine Church of God in Christ | 501(c)(3) | 2010 | | | |
| Two Rivers Church | 501(c)(3) | 2001 | | | |
| Union Baptist Church of Des Moines Iowa | 501(c)(3) | 2009 | | | |
| Union Church | 501(c)(3) | 1972 | | | |
| Well Spring Community Church | 501(c)(3) | 1996 | | | |
| Wendell Anthony Beets Foundation | 501(c)(3) | 2026 | | | |
| White Dove Ministries Inc | 501(c)(3) | 2016 | | | |
| Winners Circle Christian Church | 501(c)(3) | 2015 | | | |
| World Outreach Church of Des Moinesinc | 501(c)(3) | 2009 | | | |
| Zita Institute for Foundations and Ethics in Leadership | 501(c)(3) | 2019 | $0 | $0 | 2023-12 |
| Zomi Christian Church of Des Moines | 501(c)(3) | 2024 | | | |