| Drake University | 501(c)(3) | 1950 | $256.3 million | $628.3 million | 2025-06 |
| Grand View University | 501(c)(3) | 1939 | $66.1 million | $116.5 million | 2025-06 |
| P E O Foundation | 501(c)(3) | 1962 | $21 million | $167.8 million | 2025-06 |
| Grand View Christian School Association Inc | 501(c)(3) | 2014 | $13 million | $15.6 million | 2025-07 |
| Isu Veterinary Services Corporation | 501(c)(3) | 2013 | $6.6 million | $8 million | 2025-06 |
| Iowa Medical Education Collaborative | 501(c)(3) | 2014 | $5.9 million | $0 | 2025-06 |
| P E O Educational Loan Fund | 501(c)(3) | 1962 | $5.3 million | $99.2 million | 2025-06 |
| Joshua Christian Academy Inc | 501(c)(3) | 2012 | $3.9 million | $3.9 million | 2025-12 |
| P E O Star Scholarship | 501(c)(3) | 2010 | $3 million | $10.2 million | 2025-06 |
| P E O Scholar Awards | 501(c)(3) | 1996 | $2.9 million | $13.1 million | 2025-06 |
| Horizon Science Academy Des Moines | 501(c)(3) | 2023 | $2.5 million | $1.6 million | 2025-06 |
| Unitypoint Broadlawns Psychiatry Education Foundation | 501(c)(3) | 2019 | $2 million | $10,000 | 2025-06 |
| Roosevelt High School Foundation | 501(c)(3) | 1985 | $1.8 million | $3.4 million | 2025-08 |
| Hispanic Educational Resources Inc | 501(c)(3) | 2018 | $1.4 million | $846,691 | 2025-06 |
| Iowa Association of Community College Trustees | 501(c)(4) | 1971 | $1.4 million | $3.4 million | 2025-06 |
| Abraham Lincoln High School Alumni Foundation | 501(c)(3) | 1993 | $1.3 million | $4.6 million | 2026-05 |
| Iupat District Council 81 Joint Apprenticeship and Training Trust | 501(c)(3) | 2006 | $1 million | $3.2 million | 2025-06 |
| Building Fund of Cottey Jr College Intl CHPTR Peo Sisterhood | 501(c)(3) | 1960 | $857,760 | $2.2 million | 2025-06 |
| Iowa Safe Schools | 501(c)(3) | 2016 | $677,289 | $183,705 | 2025-06 |
| Iowa Golf Association Foundation | 501(c)(3) | 2010 | $657,184 | $5.6 million | 2024-12 |
| Endowment Fund of Cottey Jr College CHPTR Peo Sisterhood | 501(c)(3) | 1960 | $594,960 | $9.3 million | 2025-06 |
| American Mock Trial Association | 501(c)(3) | 1993 | $533,939 | $824,734 | 2025-06 |
| JW Reed Christian Academy Inc | 501(c)(3) | 2022 | $469,170 | $400,126 | 2024-12 |
| Iowa Dental Foundation | 501(c)(3) | 1994 | $468,926 | $1 million | 2025-06 |
| Des Moines Educational Foundation | 501(c)(3) | 2011 | $468,578 | $261,193 | 2025-06 |
| North High Alumni Foundation | 501(c)(3) | 1996 | $447,458 | $2.8 million | 2025-06 |
| Build Lincoln Higher Booster Club | 501(c)(3) | 2023 | $315,321 | $353,139 | 2026-04 |
| Plymouth Nursery School | 501(c)(3) | 1976 | $269,719 | $214,961 | 2026-06 |
| Des Moines Consistory Building Trust | 501(c)(3) | 1995 | $251,996 | $153,266 | 2024-12 |
| Des Moines North High School Booster Club | 501(c)(3) | 2015 | $243,744 | $194,462 | 2024-12 |
| Iowa Independent School Tuition Organization | 501(c)(3) | 2007 | $231,257 | $221,294 | 2025-12 |
| Grand View Christian School Foundation Inc | 501(c)(3) | 2015 | $228,784 | $4.1 million | 2025-12 |
| Rural School Advocates of Iowa | 501(c)(4) | 2014 | $219,247 | $102,896 | 2025-06 |
| Urban Education Network of Iowa | 501(c)(4) | 2021 | $212,303 | $262,719 | 2025-06 |
| B Well Foundation Inc | 501(c)(3) | 2021 | $209,236 | $14,985 | 2024-06 |
| Iowa Association of Christian Schools | 501(c)(4) | 2019 | $205,486 | $154,326 | 2025-06 |
| School of Mary-Montessori | 501(c)(3) | 2013 | $196,878 | $119,231 | 2024-12 |
| Empigo Academy Inc | 501(c)(3) | 2024 | $183,107 | $17,145 | 2025-06 |
| Everybody Wins-Iowa Inc | 501(c)(3) | 2004 | $180,819 | $152,728 | 2025-06 |
| Jewels Academy | 501(c)(3) | 2013 | $179,244 | $126,578 | 2025-12 |
| Westside Early Education | 501(c)(3) | 2013 | $167,016 | $1 | 2026-06 |
| Agc of Iowa Foundation | 501(c)(3) | 1997 | $163,152 | $2.1 million | 2025-12 |
| Iowans Supporting Israel Inc | 501(c)(3) | 2020 | $150,732 | $118,304 | 2025-06 |
| Central Iowa Cement Mason Apprenticeship and Training Trust | 501(c)(3) | 2009 | $140,462 | $427,467 | 2025-06 |
| Great Public Schools for Iowa | 501(c)(3) | 2023 | $127,039 | $60,830 | 2025-12 |
| Scarlet Pride Inc | 501(c)(3) | 2005 | $101,440 | $43,203 | 2024-12 |
| Serve Africa | 501(c)(3) | 2018 | $97,144 | $15,664 | 2024-12 |
| Saydel Eagles Athletic Booster Club | 501(c)(3) | 1998 | $96,508 | $131,383 | 2024-08 |
| Iowa Alliance for Choice in Education | 501(c)(3) | 2006 | $87,811 | $108,695 | 2024-12 |
| Investing in My Future Inc | 501(c)(3) | 2011 | $79,125 | $134,888 | 2025-09 |
| Clinical Legal Education Association | 501(c)(3) | 2014 | $73,005 | $284,679 | 2025-12 |
| Iowa Podiatric Medical Society Foundation | 501(c)(3) | 1991 | $67,929 | $608,705 | 2025-04 |
| Joo Hyock Incorporated | 501(c)(3) | 1983 | $30,473 | $173,575 | 2026-06 |
| Home Builders Association of Iowa Educational Corporation | 501(c)(3) | 2003 | $25,791 | $154,788 | 2025-12 |
| Des Moines Coin Club Inc | 501(c)(3) | 2014 | $22,899 | $27,720 | 2024-12 |
| Abraham Lincoln High School Alumni Association | 501(c)(3) | 1995 | $0 | $0 | 2025-12 |
| Adult Education Organization | 501(c)(3) | 2023 | $0 | $0 | 2025-03 |
| Alpha Sigma Kappa Educational Foundation | 501(c)(3) | 2017 | $0 | $0 | 2026-06 |
| American Family Thrift Store for the Blind Inc | 501(c)(3) | 2005 | | | 2010-06 |
| Band of Iron Lions | 501(c)(3) | 2022 | $0 | $0 | 2025-12 |
| Bettering Youth Organization | 501(c)(3) | 2022 | $0 | $0 | 2025-12 |
| Birds & Bees Urban Farm | 501(c)(3) | 2019 | $0 | $0 | 2025-12 |
| Black Iowa and the Nation Foundation | 501(c)(3) | 2025 | | | |
| Blades Apprenticeship Corporation | 501(c)(3) | 2025 | | | |
| Bridging the Divide Foundation | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Bright Foundation | 501(c)(3) | 2019 | | $32.7 million | 2025-06 |
| Cards Club Inc | 501(c)(3) | 2021 | $0 | $0 | 2025-12 |
| Central Iowa Beekeepers Association | 501(c)(3) | 2022 | $0 | $0 | 2025-12 |
| Central Iowa Study Club | 501(c)(3) | 2023 | $0 | $0 | 2025-12 |
| Chess It Up Iowa | 501(c)(3) | 2018 | $0 | $0 | 2024-12 |
| Child Relief Foundation Inc | 501(c)(3) | 2014 | $0 | $0 | 2024-12 |
| Christ Apostolic Temple Inc Fellowship Organization | 501(c)(3) | 2004 | | | |
| Citizens for Community Betterment | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Citizens for School Betterment Committee | 501(c)(4) | 2025 | | | |
| Comparative and Continental Philosophy Circle | 501(c)(3) | 2020 | $0 | $0 | 2025-12 |
| Cornell Parent Teacher Organization | 501(c)(3) | 2014 | $0 | $0 | 2025-08 |
| DSM Book Festival | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| Daniel Gardiner Accounting Scholarship Fund | 501(c)(3) | 2012 | | $220 | 2025-12 |
| Des Moines Business Education Alliance Inc | 501(c)(3) | 1993 | $0 | $0 | 2024-06 |
| Des Moines Neighborhood Resource Office | 501(c)(3) | 2001 | | | |
| Destiny Academy Foundation | 501(c)(3) | 2026 | | | |
| Drake University Sigma Chi House Corporation | 501(c)(7) | 2002 | $0 | $0 | 2025-07 |
| Duke Chinese Student and Scholar Helping Fundation | 501(c)(3) | 2014 | $0 | $0 | 2025-12 |
| East High Alumni Association | 501(c)(3) | 2022 | $0 | $0 | 2025-12 |
| Effort Creates Opportunity | 501(c)(3) | 2025 | | | |
| FBC Math and Science Academy | 501(c)(3) | 2010 | $0 | $0 | 2025-12 |
| Fairmount Education Inc | 501(c)(3) | 2007 | | | |
| Findley Elementary Pto | 501(c)(3) | 2023 | | | |
| Friends of the North Side Library | 501(c)(3) | 1984 | $0 | $0 | 2025-12 |
| Gamma Iota Sigma Chi Chapter - Drake | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Global Secondary Education Fund | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| Hawkeye State Fire Safety Assoc | 501(c)(3) | 2023 | $0 | $0 | 2025-12 |
| Hoover High School-Des Moines-Class of 1981 | 501(c)(7) | 2002 | $0 | $0 | 2025-12 |
| Hoover-Meredith Learning Community Foundation | 501(c)(3) | 2009 | $0 | $0 | 2026-06 |
| Hoyt Middle School Pto | 501(c)(3) | 2024 | $0 | $0 | 2026-06 |
| International Association for Japanese Philosophy | 501(c)(3) | 2020 | $0 | $0 | 2025-12 |
| Iowa Association of Alternative Education | 501(c)(3) | 2026 | | | |
| Iowa Association of School Boards Foundation Inc | 501(c)(3) | 2001 | $0 | $0 | 2025-06 |
| Iowa Centennial Memorial Foundation | 501(c)(3) | 1961 | $0 | $0 | 2025-05 |
| Iowa Coalition of Recovery Support Providers | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Iowa Figure Skating School | 501(c)(3) | 2020 | $0 | $0 | 2025-12 |
| Iowa Horizon Science Academy | 501(c)(3) | 2024 | $0 | $0 | 2025-03 |
| Iowa Insurance Day Foundation | 501(c)(3) | 2023 | $0 | $0 | 2025-06 |
| Iowa Literacy Council | 501(c)(3) | 2017 | $0 | $0 | 2025-12 |
| Iowa Talented and Gifted | 501(c)(3) | 1980 | $0 | $209,443 | 2025-12 |
| Iowans Against the Death Penalty Fund | 501(c)(3) | 2019 | $0 | $0 | 2026-06 |
| Iron Warriors MC Crued Pigs Central Iowa Chapter Inc | 501(c)(3) | 2011 | $0 | $0 | 2025-12 |
| Jack D Levin Family Law American Inns of Court for Greater | 501(c)(3) | 2025 | $0 | $0 | 2025-06 |
| K9 Alliance of Iowa | 501(c)(3) | 2009 | $0 | $0 | 2025-12 |
| Kansas Beta Educational Foundation | 501(c)(3) | 2007 | $0 | $0 | 2025-12 |
| Kids Foundation International | 501(c)(3) | 2020 | $0 | $0 | 2025-12 |
| Latinos Unidos of Iowa | 501(c)(3) | 2012 | $0 | $0 | 2026-03 |
| Les Dames Descoffier Greater Des Moines Chapter Inc | 501(c)(3) | 2017 | $0 | $0 | 2025-12 |
| Lincoln High School Band Boosters | 501(c)(3) | 2019 | $0 | $0 | 2026-06 |
| Lincoln Vocal Music Boosters | 501(c)(3) | 2024 | | | |
| Little Big Women | 501(c)(3) | 2019 | $0 | $0 | 2025-12 |
| Living Oaks Cottage School | 501(c)(3) | 2026 | | | |
| Mary and Mike Earley Foundation | 501(c)(3) | 2026 | | | |
| Merrill Middle School Pta | 501(c)(3) | 2015 | $0 | $0 | 2024-06 |
| Mflmarmac Education Association | 501(c)(5) | 1997 | $0 | $0 | 2025-08 |
| Mid Iowa Woodcarvers Inc | 501(c)(3) | 2026 | $0 | $0 | 2025-12 |
| Movement for the Promotion of Gender Equailty in Liberia | 501(c)(3) | 2014 | $0 | $0 | 2026-06 |
| Mu Omicron Foundation | 501(c)(3) | 2002 | $0 | $0 | 2025-12 |
| Naples Macfriends User Group Inc | 501(c)(3) | 2011 | $0 | $0 | 2025-12 |
| National Assistance Foundation | 501(c)(3) | 2013 | | | 2018-12 |
| National Association of Teachers of Singing Inc | 501(c)(3) | 2009 | $0 | $0 | 2025-12 |
| National Institute of Governmental Purchasing Inc | 501(c)(3) | 1945 | $0 | $0 | 2025-12 |
| Norah Kudee Foundation Inc | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| North American Levinas Society | 501(c)(3) | 2012 | $0 | $0 | 2026-01 |
| North High Alumni Association | 501(c)(3) | 1996 | $0 | $0 | 2026-06 |
| Oaks of Redemption Inc | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Optasia Ministry Inc | 501(c)(3) | 2007 | $0 | $0 | 2025-12 |
| Parents & Friends of Moore Elementary | 501(c)(3) | 2016 | $0 | $0 | 2025-12 |
| Penelope 38 Charitable Trust | 501(c)(3) | 1998 | | $1,695 | 2024-12 |
| Perkins Elementary Pto | 501(c)(3) | 2026 | | | |
| Phi Eta Sigma National Honor Society Inc | 501(c)(3) | 2014 | | | |
| Pioneers Trust Foundation | 501(c)(3) | 2011 | $0 | $0 | 2025-08 |
| Postsecondary Attainment Institute | 501(c)(3) | 2023 | $0 | $0 | 2025-06 |
| Pta Iowa Congress | 501(c)(3) | 2023 | $0 | $0 | 2026-04 |
| Read Anyways | 501(c)(3) | 2025 | | | |
| Rich Eychaner Charitable Foundation | 501(c)(3) | 1988 | | $16,630 | 2025-11 |
| Samuelson Elementary School Parent Teacher Organization | 501(c)(3) | 2008 | $0 | $0 | 2025-06 |
| Saydel Alumni Association | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Saydel Eagle Endowment | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Scottish Rite Orient of Iowa | 501(c)(3) | 2003 | $0 | $0 | 2024-12 |
| Sebastian Kidd Foundation Inc | 501(c)(3) | 2022 | | $2,179 | 2025-04 |
| Sigma Chi Fraternity Theta Gamma Chapter | 501(c)(7) | 2019 | $0 | $0 | 2025-07 |
| Six Degrees of Separation-Removing Barriers for Education | 501(c)(3) | 2025 | $0 | $0 | 2025-06 |
| Society for Annuity Facts & Education Safe Inc | 501(c)(3) | 2014 | $0 | $0 | 2025-12 |
| Stem Simulations | 501(c)(3) | 2023 | $0 | $0 | 2025-12 |
| Studebaker Pto Inc | 501(c)(3) | 2024 | | | |
| The Cressant Foundation | 501(c)(3) | 1995 | | $2.7 million | 2025-12 |
| The Iowa Catholic Scholarship Granting Organization | 501(c)(3) | 2026 | | | |
| The Royal Ballet of Iowa | 501(c)(3) | 2025 | | | |
| Titans of the Midwest | 501(c)(3) | 2017 | $0 | $0 | 2025-12 |
| United Upper Nile South Sudanese Community Association in Iowa | 501(c)(3) | 2017 | $0 | $0 | 2025-12 |
| Us Education Without Borders Foundation | 501(c)(3) | 2021 | | $7,215 | 2025-11 |
| Warwick | 501(c)(3) | 2023 | $0 | $0 | 2025-12 |