| Christian & Missionary Alliance Foundation Inc | 501(c)(3) | 1967 | $159.7 million | $1.02 billion | 2025-06 |
| Cypress Cove at Healthpark Florida Inc | 501(c)(3) | 1996 | $65.6 million | $303 million | 2025-09 |
| Child Care of Southwest Florida Inc | 501(c)(3) | 1967 | $16.1 million | $6 million | 2025-06 |
| Childrens Advocacy Center of SW Florida Inc | 501(c)(3) | 1989 | $7.3 million | $8 million | 2025-06 |
| Community Cooperative Inc | 501(c)(3) | 1985 | $6 million | $6.7 million | 2025-12 |
| Shalimare Incorporated | 501(c)(3) | 2010 | $5.8 million | $2.4 million | 2025-04 |
| Valeries House Inc | 501(c)(3) | 2015 | $3.4 million | $6.9 million | 2024-12 |
| Interfaith Charities of South Lee Inc | 501(c)(3) | 1993 | $2.8 million | $961,514 | 2025-12 |
| Charity Pros Inc | 501(c)(3) | 2019 | $2 million | $2.4 million | 2024-12 |
| Our Mothers Home of Southwest Florida Inc | 501(c)(3) | 1994 | $1.5 million | $2.3 million | 2024-12 |
| Dr Piper Center for Social Services Inc | 501(c)(3) | 1998 | $1.5 million | $1 million | 2025-09 |
| Source of Light and Hope Development Center Inc | 501(c)(3) | 1988 | $1.1 million | $316,933 | 2024-12 |
| Jungle Kids for Christ Inc | 501(c)(3) | 2012 | $905,704 | $956,373 | 2024-12 |
| Project Dentist Care Inc | 501(c)(3) | 2021 | $845,458 | $581,506 | 2025-06 |
| Regenerate Your Authentic Nature Inc | 501(c)(3) | 2014 | $528,254 | $1.4 million | 2025-12 |
| Trailways Camps Inc | 501(c)(3) | 2022 | $443,469 | $128,087 | 2025-06 |
| New Life Centers | 501(c)(3) | 2014 | $412,570 | $227,628 | 2025-12 |
| Hope 4 Kids Incorporated | 501(c)(3) | 2018 | $341,625 | $6,438 | 2024-12 |
| Imagine Initiative Inc | 501(c)(3) | 2022 | $302,604 | $58,216 | 2025-12 |
| Childrens Kingdom Foundation Inc | 501(c)(3) | 2021 | $280,174 | $10,821 | 2025-12 |
| Home Front Heroes Inc | 501(c)(3) | 2022 | $254,646 | $1.5 million | 2025-12 |
| Christian Medical Ministries | 501(c)(3) | 2015 | $242,173 | $50,668 | 2025-12 |
| The Freedom & Virtue Institute Inc | 501(c)(3) | 2009 | $214,403 | $189,646 | 2025-12 |
| Military Spouse Corporate Career Network Inc | 501(c)(3) | 2007 | $188,014 | $1.4 million | 2025-12 |
| Providence Family Life Center | 501(c)(3) | 2015 | $144,453 | $30,894 | 2025-12 |
| Southwest Florida Gwi Housing Inc | 501(c)(3) | 1996 | $136,136 | $735,361 | 2025-12 |
| Family Resource Center of Southwest Florida Inc | 501(c)(3) | 2010 | $95,943 | $155,228 | 2024-12 |
| Southwest Florida Gwi Housing III Inc | 501(c)(3) | 1998 | $95,045 | $503,779 | 2025-12 |
| Tiny Showers Inc | 501(c)(3) | 2022 | $93,999 | $33,664 | 2025-12 |
| Southwest Florida Gwi Housing IX Inc | 501(c)(3) | 2002 | $85,001 | $300,575 | 2025-12 |
| After the Rain of SW Florida Inc | 501(c)(3) | 2006 | $73,349 | $257,751 | 2025-12 |
| Gujarati Samaj of Southwest Florida Inc | 501(c)(3) | 2016 | $53,354 | $232,093 | 2024-12 |
| Visions of Sugar Plums | 501(c)(3) | 2022 | $53,241 | $1,629 | 2025-12 |
| Local 1826 Leroy Nottingham Firefighter Paramedic Benevolent F | 501(c)(3) | 2019 | $43,585 | $125,485 | 2025-12 |
| Reflective Spaces Ministry Corp | 501(c)(3) | 2021 | $35,966 | $73,928 | 2025-12 |
| Alienated Parent Action & Resource Team Inc | 501(c)(3) | 2023 | $9,404 | $3,125 | 2024-12 |
| A Better Community Vision Center Inc | 501(c)(3) | 2022 | $5,000 | $1 | 2025-12 |
| Sandy Beaches Foundation Corporation | 501(c)(3) | 2023 | $3,320 | $919 | 2024-12 |
| Acf Direct Impact Foundation Inc | 501(c)(3) | 2024 | | | |
| All Saints Childrens Foundation Inc | 501(c)(3) | 2025 | | | |
| Alva Angels | 501(c)(3) | 2014 | $0 | $0 | 2025-07 |
| Archangel Raphael Ministry Center Inc | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Arl Charities Inc | 501(c)(3) | 2026 | | | |
| Autumns Gifts of Hope Inc | 501(c)(3) | 2021 | $0 | $0 | 2025-12 |
| Blankets and Blessings Inc | 501(c)(3) | 2015 | $0 | $0 | 2025-12 |
| Blind Sailing Unlimited Inc | 501(c)(3) | 2023 | $0 | $0 | 2025-12 |
| Breaking Free Living Well Corp | 501(c)(3) | 2025 | $0 | $0 | 2026-01 |
| Buckingham Community Association Inc | 501(c)(3) | 2024 | $0 | $0 | 2024-12 |
| CCS Food & Resources Foundation Corp | 501(c)(3) | 2025 | | | |
| Catalyst for the World | 501(c)(3) | 2025 | | | |
| Chosen Youth 2 Group Home Inc | 501(c)(3) | 2025 | | $30,835 | 2025-12 |
| Community Center for Social Integration | 501(c)(3) | 2022 | $0 | $0 | 2024-12 |
| Compassionate Care 4 You Inc | 501(c)(3) | 2022 | $0 | $0 | 2025-12 |
| Connected Families Collective Inc | 501(c)(3) | 2026 | | | |
| Covenant Homes of Hope | 501(c)(3) | 2022 | $0 | $0 | 2025-12 |
| Deaf Empowering Advocating Florida Southwest | 501(c)(3) | 2023 | $0 | $534 | 2024-12 |
| Dibenedetto Family Foundation Inc | 501(c)(3) | 2024 | | $2.6 million | 2024-12 |
| Dunbar Community Outreach Network Inc | 501(c)(3) | 2021 | $0 | $0 | 2025-12 |
| Dunbar Gospel Association of Southwest Florida Inc | 501(c)(3) | 2003 | $0 | $0 | 2026-03 |
| Dunham Family Foundation Inc | 501(c)(3) | 2023 | | $1 | 2025-12 |
| Edison Kiwanis Club Foundation Inc | 501(c)(3) | 2017 | $0 | $0 | 2025-09 |
| Family Connect Inc | 501(c)(3) | 2022 | $0 | $0 | 2025-08 |
| Fathers Inspiring Strength & Hope I NC | 501(c)(3) | 2026 | | | |
| Fisher Foundation Inc | 501(c)(3) | 2026 | | | |
| Focus Foundation of Florida Inc | 501(c)(3) | 2025 | | | |
| Fort Counseling Center Inc | 501(c)(3) | 2026 | | | |
| Fundacion Palabra Viva Inc | 501(c)(3) | 2025 | | | |
| Fundacion Su Fortaleza Mi Refugio Inc | 501(c)(3) | 2022 | | $1 | 2025-12 |
| Game Day Bunch Inc | 501(c)(3) | 2016 | $0 | $0 | 2024-12 |
| Girl Me Too Inc | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| Gods Divine Design Inc | 501(c)(3) | 2003 | $0 | $0 | 2024-12 |
| Gods Giving Hands Incorporated | 501(c)(3) | 2014 | $0 | $0 | 2025-12 |
| Grieving Mothers Against Murders Inc | 501(c)(3) | 2022 | $0 | $0 | 2025-12 |
| Gulf Harbour Charitable Foundation Inc | 501(c)(3) | 2023 | | $143,240 | 2025-12 |
| Halo Project of Southwest Florida Inc | 501(c)(3) | 2022 | $0 | $0 | 2025-12 |
| Hands 2 Help Charitable Inc | 501(c)(3) | 2026 | $0 | $0 | 2025-10 |
| Happy Backpack Inc | 501(c)(3) | 2024 | | | |
| Haven of Hope SWF Inc | 501(c)(3) | 2024 | | | |
| Helping Those Who Help Themselves | 501(c)(3) | 2023 | $0 | $0 | 2025-12 |
| Her Homes Inc | 501(c)(3) | 2023 | $0 | $0 | 2025-12 |
| Heritage Palms Charitable Organization Inc | 501(c)(3) | 2022 | | $88,924 | 2025-12 |
| Holiday House and Stroll of Fort Myers | 501(c)(3) | 2026 | | | |
| Hope By Song | 501(c)(3) | 2015 | $0 | $0 | 2024-12 |
| Hopes Heart for Healing Inc | 501(c)(3) | 2022 | $0 | $0 | 2024-12 |
| Jonathans Place of SWFL Inc | 501(c)(3) | 2021 | $0 | $0 | 2024-12 |
| Kids Give | 501(c)(3) | 2021 | | $1 | 2025-12 |
| Kids Way | 501(c)(3) | 2016 | $0 | $0 | 2025-12 |
| Kind Heart Projects Corporation | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Legacymakers Foundation | 501(c)(3) | 2019 | $0 | $0 | 2024-12 |
| Lets Pass Inc | 501(c)(3) | 2025 | | | |
| Lien Sacre Inc | 501(c)(3) | 2026 | | | |
| Living in Deliverance International Ministry | 501(c)(3) | 2012 | $0 | $0 | 2025-12 |
| Lolo Foundation Incorporated | 501(c)(3) | 2023 | $0 | $0 | 2024-12 |
| Love All Feed All Inc | 501(c)(3) | 2022 | $0 | $0 | 2025-12 |
| My Name My Voice | 501(c)(3) | 2026 | | | |
| National Coalition for Patriots Inc | 501(c)(3) | 2022 | $0 | $0 | 2025-12 |
| Neighbors Network of Lee County | 501(c)(3) | 2022 | $0 | $0 | 2025-12 |
| New Beginnings MSB Inc | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Old Florida Dream Team Foundation | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Orphan Support International Inc | 501(c)(3) | 2019 | $0 | $0 | 2026-06 |
| PTSD Awareness Summit | 501(c)(3) | 2020 | $0 | $0 | 2025-12 |
| Path Family Foundations Inc | 501(c)(3) | 2026 | | | |
| Patriot Cruise and Salute | 501(c)(3) | 2009 | $0 | $0 | 2025-12 |
| Pawsitive Kids Camp Inc | 501(c)(3) | 2015 | $0 | $0 | 2026-06 |
| Pennies for Community Progress of Lee County Inc | 501(c)(4) | 2016 | $0 | $0 | 2024-06 |
| Quest Ecss Corp | 501(c)(3) | 2017 | $0 | $0 | 2025-12 |
| Resource de Layor Inc | 501(c)(3) | 2023 | $0 | $0 | 2025-12 |
| SD Marsland & Partners Inc | 501(c)(3) | 2024 | | | |
| Salsabil Charitable Corporation | 501(c)(3) | 2012 | | $72,176 | 2024-12 |
| Salvation Halfway House Ministries Inc | 501(c)(3) | 2020 | $0 | $0 | 2025-11 |
| San Carlos Haitian Baptist Church Inc | 501(c)(3) | 2024 | | | |
| Santa Claus Society Inc | 501(c)(3) | 2021 | $0 | $0 | 2025-12 |
| Sew & Sews | 501(c)(3) | 2021 | $0 | $0 | 2025-12 |
| Share My Love Inc | 501(c)(3) | 2024 | $0 | $0 | 2025-01 |
| Silent Giving Society | 501(c)(3) | 2026 | | | |
| Silver Years Foundation | 501(c)(3) | 2023 | $0 | $0 | 2025-12 |
| Sound70 Org Inc | 501(c)(3) | 2021 | $0 | $0 | 2025-12 |
| Southern Fresh Urban Farm Collective Inc | 501(c)(3) | 2022 | $0 | $0 | 2025-12 |
| Steadfast Faith Ministries Inc | 501(c)(3) | 2019 | $0 | $0 | 2024-12 |
| Step-By-Step Family Foundation Inc | 501(c)(3) | 2026 | | | |
| The Quest Ecss 2 | 501(c)(3) | 2026 | | | |
| The Socorro Foundation Inc | 501(c)(3) | 2026 | | | |
| Toluwani Helping Hands Inc | 501(c)(3) | 2008 | $0 | $0 | 2024-12 |
| Trivial Blue Dot Inc | 501(c)(3) | 2021 | $0 | $0 | 2025-12 |
| Unlimited Dreams To Reality | 501(c)(3) | 2021 | $0 | $0 | 2025-12 |
| Viles & Beckman Foundation Inc | 501(c)(3) | 2023 | $0 | $0 | 2024-12 |
| Visuality Inc | 501(c)(3) | 2011 | $0 | $0 | 2024-06 |
| Widows Sons of Florida Masonic Riders Association Inc | 501(c)(3) | 2015 | $0 | $0 | 2024-12 |