| Lutheran Charity Association | Health care | 501(c)(3) | 1948 | $84.3 million | $107.8 million | 2025-06 |
| First Community Credit Union | | 501(c)(14) | 1971 | $76.7 million | $1.33 billion | 2024-12 |
| University of Jamestown | Education | 501(c)(3) | 1937 | $67.5 million | $153 million | 2025-06 |
| Anne Carlsen Center | Education | 501(c)(3) | 2004 | $48.4 million | $169.9 million | 2025-12 |
| Anne Carlsen Center Foundation | Human services | 501(c)(3) | 2004 | $7.7 million | $96.4 million | 2025-12 |
| Community Action Region Vi | Housing and shelter | 501(c)(3) | 1978 | $4.9 million | $1.9 million | 2025-12 |
| Progress Enterprises Inc | | 501(c)(3) | 1976 | $4 million | $6.3 million | 2025-06 |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1966 | $3.8 million | $124.2 million | 2025-09 |
| Alpha Opportunities Inc | Human services | 501(c)(3) | 1980 | $3.4 million | $3.3 million | 2023-06 |
| Victory Christian School | Education | 501(c)(3) | 2009 | $3.3 million | $2.9 million | 2024-12 |
| James River Senior Citizens Center Inc | | 501(c)(3) | 1970 | $2.6 million | $4 million | 2024-12 |
| Jamestown Stutsman Development Corporation | | 501(c)(4) | 1992 | $1.7 million | $18.2 million | 2025-12 |
| Jamestown Regional Medical Center Foundation Inc | Health care | 501(c)(3) | 1985 | $1.3 million | $7.3 million | 2025-06 |
| Jamestown Parks and Recreation Foundation Inc | Recreation and sports | 501(c)(3) | 2006 | $1 million | $970,447 | 2025-12 |
| Jamestown College Foundation Inc | | 501(c)(3) | 1986 | $933,736 | $20.4 million | 2025-06 |
| Jamestown Country Club | | 501(c)(7) | 1979 | $903,739 | $1.6 million | 2025-12 |
| Guardian Angels Inc | Health care | 501(c)(3) | 2015 | $897,469 | $369,016 | 2024-12 |
| Project Apis M 2 0 Inc | Animal-related | 501(c)(3) | 2018 | $874,379 | $833,874 | 2025-06 |
| Jamestown Tourism | Community improvement and capacity building | 501(c)(6) | 2004 | $765,333 | $753,129 | 2024-12 |
| Science & Environmental Health Network | Environment | 501(c)(3) | 1999 | $718,608 | $557,719 | 2025-12 |
| Jamestown Fine Arts Association Incorporated | | 501(c)(3) | 1982 | $671,844 | $2.9 million | 2025-06 |
| Vets Club Inc | Public and societal benefit | 501(c)(19) | 1999 | $666,936 | $556,603 | 2024-12 |
| Jamestown Area Chamber of Commerce | | 501(c)(6) | 1934 | $662,940 | $476,951 | 2024-12 |
| Jamestown Hockey Booster Club Inc | Recreation and sports | 501(c)(3) | 1986 | $600,703 | $918,039 | 2026-04 |
| North American Bison Discovery Center | Arts, culture and humanities | 501(c)(3) | 1992 | $598,062 | $1.3 million | 2025-12 |
| Youth for Christ USA Inc | | 501(c)(3) | 1954 | $557,969 | $2.4 million | 2025-06 |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1966 | $532,847 | $2.2 million | 2025-09 |
| Face | Education | 501(c)(3) | 1977 | $447,376 | $3.5 million | 2025-12 |
| Jamestown Gymnastics Club | Recreation and sports | 501(c)(3) | 1985 | $382,228 | $1.3 million | 2025-07 |
| North Dakota Farmers Union Educational Foundation | Food, agriculture and nutrition | 501(c)(3) | 2003 | $374,454 | $2.6 million | 2025-09 |
| Bridgewell | Human services | 501(c)(3) | 2024 | $373,585 | $0 | 2026-04 |
| South Central Dakota Regional Council | Community improvement and capacity building | 501(c)(3) | 2018 | $365,214 | $1.6 million | 2025-03 |
| S A F E Shelter | | 501(c)(3) | 1980 | $356,649 | $1.7 million | 2025-06 |
| James River Humane Society Inc | Animal-related | 501(c)(3) | 1986 | $349,810 | $523,606 | 2026-03 |
| Family Support Network of North Dakota Inc | | 501(c)(3) | 1985 | $332,245 | $546,146 | 2025-12 |
| Prairie Leadership Partners | Public and societal benefit | 501(c)(4) | 2025 | $320,050 | $86,896 | 2025-12 |
| USA Softball of North Dakota | | 501(c)(3) | 1985 | $311,270 | $333,704 | 2025-12 |
| Jamestown Frontier Attractions | Education | 501(c)(3) | 2025 | $225,539 | $245,470 | 2025-12 |
| Highland Cemetery Association | Mutual and membership benefit | 501(c)(13) | 1964 | $210,491 | $1 million | 2025-12 |
| North Dakota Farmers Union Foundation | Community improvement and capacity building | 501(c)(3) | 2000 | $198,163 | $362,642 | 2025-09 |
| St Pauls United Methodist Church Endowment Foundation | | 501(c)(3) | 1992 | $197,386 | $1.1 million | 2025-12 |
| Jamestown United Way | Philanthropy, voluntarism and grantmaking foundations | 501(c)(3) | 1971 | $191,783 | $270,683 | 2025-04 |
| Jamestown Area Youth BB | Recreation and sports | 501(c)(3) | 2011 | $188,053 | $76,371 | 2024-12 |
| Benevolent & Protective Order of Elks of the USA | | 501(c)(8) | 1951 | $174,421 | $562,581 | 2026-03 |
| Gordon A Larson Foundation Inc | Philanthropy, voluntarism and grantmaking foundations | 501(c)(3) | 2003 | $153,918 | $2.5 million | 2025-12 |
| Pacific Association for Medical Equipment Services | Health care | 501(c)(6) | 1983 | $134,604 | $45,786 | 2025-12 |
| North Dakota Music Educators Association | Arts, culture and humanities | 501(c)(3) | 2000 | $133,029 | $69,640 | 2025-06 |
| Jamestown Ems Education Inc | Health care | 501(c)(3) | 2000 | $122,798 | $110,585 | 2025-12 |
| Jamesriver Figure Skating Club Inc | Recreation and sports | 501(c)(3) | 1997 | $116,656 | $76,365 | 2026-04 |
| Jamestown Regional Entrepreneur Center | Community improvement and capacity building | 501(c)(3) | 2018 | $114,318 | $120,611 | 2025-12 |
| Jamestown Rural Fire Department | Public safety, disaster preparedness and relief | 501(c)(3) | 2017 | $110,422 | $469,205 | 2025-12 |
| McCoy Ministries | Religion-related | 501(c)(3) | 1994 | $102,151 | $72,020 | 2025-12 |
| Nierling Family Scholarship Jamestown College Trust | Education | 501(c)(3) | 2001 | $85,688 | $849,709 | 2025-12 |
| Jamestown Soccer Federation | | 501(c)(3) | 1983 | $80,656 | $64,585 | 2024-12 |
| Firm Foundations | Mental health and crisis intervention | 501(c)(3) | 2019 | $71,827 | $2,500 | 2025-12 |
| Jamestown Community Foundation | | 501(c)(3) | 1986 | $69,727 | $1.1 million | 2025-12 |
| Jamestown Basketball Boosters | Recreation and sports | 501(c)(3) | 1995 | $67,771 | $74,400 | 2024-12 |
| James River Rodeo Inc | Recreation and sports | 501(c)(4) | 2011 | $64,815 | $19,183 | 2024-12 |
| Jamestown Cancer Support Group | Diseases, disorders and medical disciplines | 501(c)(3) | 2018 | $64,032 | $84,199 | 2025-12 |
| North Dakota Envirothon | Education | 501(c)(3) | 2018 | $62,948 | $64,576 | 2025-12 |
| Stutsman County Wildlife Federation | Animal-related | 501(c)(3) | 1990 | $57,862 | $193,773 | 2025-12 |
| James Valley Robotics Association | Human services | 501(c)(3) | 2021 | $51,423 | $32,670 | 2025-06 |
| Prairie Paws Rescue | Animal-related | 501(c)(3) | 2008 | $48,757 | $312,021 | 2025-06 |
| Energy Share of North Dakota Inc | | 501(c)(3) | 1987 | $43,129 | $162,328 | 2025-12 |
| JHS Sophistikix Booster Club | Recreation and sports | 501(c)(3) | 2018 | $31,513 | $5,384 | 2025-12 |
| Nierling-United Way Trust | Philanthropy, voluntarism and grantmaking foundations | 501(c)(3) | 2001 | $2,349 | $86,953 | 2024-12 |
| Aglow International | | 501(c)(3) | 1973 | $0 | $0 | 2021-12 |
| Alano Society | | 501(c)(3) | 1981 | $0 | $0 | 2024-12 |
| American Association of University Women Jamestown Branch | | 501(c)(4) | 2010 | $0 | $0 | 2026-06 |
| American Legion | | 501(c)(19) | 1946 | $0 | $0 | 2025-12 |
| American Legion Auxiliary | | 501(c)(19) | 1946 | $0 | $0 | 2026-06 |
| American String Teachers Association Incorporated | | 501(c)(3) | 1988 | $0 | $0 | 2026-06 |
| Ancient Free & Accepted Masons of N D | | 501(c)(8) | 1952 | $0 | $0 | 2025-12 |
| Association for Educ and Rehab of Blind and Visually Impaired Dakotas Chap | Diseases, disorders and medical disciplines | 501(c)(3) | 2018 | $0 | $0 | 2025-12 |
| Ave Marie Village | Health care | 501(c)(3) | 1946 | | | |
| Bahais of Jamestown | | 501(c)(3) | 1995 | | | |
| Birthday Blessings | Human services | 501(c)(3) | 2023 | $0 | $0 | 2025-12 |
| Bison World Fund | Animal-related | 501(c)(3) | 2023 | $0 | $0 | 2023-12 |
| Blue Jay Girls Hockey Boosters | Recreation and sports | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Blue Jay Volleyball Boosters Inc | Recreation and sports | 501(c)(3) | 2011 | $0 | $0 | 2024-12 |
| Bluegrass Association of North Dakota | Arts, culture and humanities | 501(c)(3) | 2012 | $0 | $0 | 2025-12 |
| Borges Family Way | Human services | 501(c)(3) | 2016 | $0 | $0 | 2025-12 |
| Brotherhood of Maintenance of Way Employees | | 501(c)(5) | 1941 | $0 | $0 | 2026-03 |
| Buffalo City Church Inc | Religion-related | 501(c)(3) | 2017 | | | |
| Camp Rokiwan Inc | | 501(c)(3) | 1975 | $0 | $0 | 2025-12 |
| Carmichael Classical Christian School | Education | 501(c)(3) | 2024 | $0 | $0 | 2026-06 |
| Circle Cross Riding Club | Animal-related | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Community Corrections Advisory Board | | 501(c)(3) | 1992 | $0 | $0 | 2025-12 |
| Concordia Lutheran Church Inc | | 501(c)(3) | 1941 | | | |
| Dakota RC | Recreation and sports | 501(c)(3) | 2026 | | | |
| Disabled American Veterans | | 501(c)(4) | 1942 | $0 | $0 | 2026-06 |
| Dorcas Society Ministry | International, foreign affairs and national security | 501(c)(3) | 2019 | $0 | $0 | 2025-12 |
| Dragon Football Parents | Recreation and sports | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Farmers EDUCATIONAL& Cooperative Union of America North Dakota | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1966 | | | |
| Farmers Educational & Cooperative Union of America North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational & Cooperative Union of American North Dakota Div | | 501(c)(5) | 1937 | | | |
| Farmers Educational and Cooperative Union of America North Dakota Div | | 501(c)(5) | 1966 | | | |
| First Assembly of God | | 501(c)(3) | 1964 | | | |
| First Baptist Church | | 501(c)(3) | 1972 | | | |
| First Community Credit Union Foundation | Community improvement and capacity building | 501(c)(3) | 2025 | | | |
| Forjamestown | Public and societal benefit | 501(c)(3) | 2023 | $0 | $0 | 2025-12 |
| Fort Seward Inc | Arts, culture and humanities | 501(c)(3) | 2024 | $0 | $0 | 2025-07 |
| Fraternal Order of Eagles | | 501(c)(8) | 1991 | $0 | $0 | 2025-05 |
| Fraternal Order of Police | | 501(c)(8) | 1952 | $0 | $101,498 | 2025-12 |
| Friends of the James River Valley Library System | Education | 501(c)(3) | 2017 | $0 | $0 | 2025-12 |
| HDM Legacy | Religion-related | 501(c)(3) | 2025 | | | |
| Healing Well Inc | Religion-related | 501(c)(3) | 2025 | | | |
| Hillcrest Adventist Elementary School | | 501(c)(3) | 1947 | | | |
| International Brotherhood of 0239 Local Union | Employment | 501(c)(5) | 2025 | $0 | $0 | 2025-06 |
| International Church of the Foursquare Gospel | | 501(c)(3) | 1949 | | | |
| Jamestown Area Christian Entertainment | Religion-related | 501(c)(3) | 2022 | $0 | $0 | 2025-12 |
| Jamestown Area Pickleball Club | Recreation and sports | 501(c)(7) | 2022 | $0 | $0 | 2025-12 |
| Jamestown Board of Realtors | | 501(c)(6) | 1967 | $0 | $0 | 2024-12 |
| Jamestown Choralaires | Arts, culture and humanities | 501(c)(3) | 2023 | $0 | $0 | 2025-07 |
| Jamestown Drag Racing Association | Arts, culture and humanities | 501(c)(4) | 2011 | $0 | $0 | 2025-12 |
| Jamestown Education Association | | 501(c)(5) | 1984 | $0 | $0 | 2025-06 |
| Jamestown Frontier Fastpitch | Recreation and sports | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| Jamestown H O G Chapter | Recreation and sports | 501(c)(7) | 2014 | $0 | $0 | 2025-12 |
| Jamestown Marksmanship Education Range Inc | Education | 501(c)(3) | 2019 | $0 | $0 | 2025-12 |
| Jamestown Masonic Foundation | Mutual and membership benefit | 501(c)(10) | 2011 | $0 | $0 | 2024-12 |
| Jamestown Medical Foundation | | 501(c)(3) | 1964 | | $201,206 | 2025-09 |
| Jamestown Middle School Archery Tea M | Recreation and sports | 501(c)(3) | 2026 | | | |
| Jamestown Nurse Honor Guard | Diseases, disorders and medical disciplines | 501(c)(3) | 2026 | | | |
| Jamestown Patriotic Council | Education | 501(c)(3) | 2024 | $0 | $0 | 2025-05 |
| Jamestown Public School Foundation | Education | 501(c)(3) | 1998 | $0 | $0 | 2025-06 |
| Jamestown Public Schools Music Boosters | Education | 501(c)(3) | 2009 | $0 | $0 | 2026-08 |
| Jamestown Sertoma Club | Philanthropy, voluntarism and grantmaking foundations | 501(c)(3) | 2014 | $0 | $0 | 2024-12 |
| Jamestown Wrestling Association | Recreation and sports | 501(c)(3) | 2023 | $0 | $0 | 2025-08 |
| Jamestown Youth Archery Club | Youth development | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| Kiwanis International Inc | | 501(c)(4) | 1940 | $0 | $0 | 2025-09 |
| Knights of Columbus | | 501(c)(8) | 1940 | $0 | $0 | 2025-12 |
| Legendary Health & Human Services | Human services | 501(c)(3) | 2025 | | | |
| Lincoln Elementary Parent Teacher Association | Education | 501(c)(3) | 2017 | $0 | $0 | 2025-12 |
| Living Is Giving Inc | Philanthropy, voluntarism and grantmaking foundations | 501(c)(3) | 2022 | $0 | $0 | 2025-12 |
| Modern Woodmen of America | | 501(c)(8) | 1961 | $0 | $0 | 2025-12 |
| Modern Woodmen of America | | 501(c)(8) | 1972 | $0 | $0 | 2025-12 |
| ND Breastfeeding Coalition | | 501(c)(3) | 2016 | $0 | $0 | 2025-12 |
| National Rural Letter Carriers Association | | 501(c)(5) | 1972 | $0 | $0 | 2026-06 |
| Nierling Anne Carlsen Center Trust | | 501(c)(92) | | | $208,500 | 2025-12 |
| Nierling Jamestown Hospital Trust | Philanthropy, voluntarism and grantmaking foundations | 501(c)(3) | 2001 | | $62,658 | 2024-12 |
| Nodak Grace | Human services | 501(c)(3) | 2023 | $0 | $0 | 2024-12 |
| North Dakota 1752 Club | Education | 501(c)(3) | 2020 | $0 | $0 | 2025-12 |
| North Dakota Amateur Baseball Inc | | 501(c)(4) | 1988 | $0 | $0 | 2025-12 |
| North Dakota Babe Ruth Baseball | Recreation and sports | 501(c)(3) | 2025 | $0 | $0 | 2025-08 |
| North Dakota Conference of Seventh Day Adventists | | 501(c)(3) | 1934 | | | |
| North Dakota Sports Hall of Fame Inc | | 501(c)(3) | 1994 | $0 | $0 | 2024-12 |
| North Dakota Travel Alliance Partnership | Community improvement and capacity building | 501(c)(6) | 2019 | $0 | $0 | 2025-12 |
| Polly Ann Neumiller Memorial Organization | Public and societal benefit | 501(c)(3) | 2021 | $0 | $0 | 2025-12 |
| Primera Iglesia Del Nazareno Church of the Nazarene | | 501(c)(3) | 1963 | | | |
| Psi Chi the National Honor Society in Psychology | | 501(c)(3) | 1957 | $0 | $0 | 2025-06 |
| Pure Hockey Skills | Youth development | 501(c)(3) | 2018 | $0 | $0 | 2024-12 |
| Region D Dac | Public and societal benefit | 501(c)(6) | 2014 | $0 | $0 | 2025-10 |
| Ronald K Carlson Jamestown Church Trust | | 501(c)(91) | | | | |
| Rotary International | | 501(c)(4) | 1966 | $0 | $0 | 2026-06 |
| Sons of Norway | | 501(c)(8) | 1940 | $0 | $0 | 2025-12 |
| South Central Homeless Coalition | Human services | 501(c)(3) | 2007 | $0 | $0 | 2025-12 |
| St James Basilica of Jamestown | | 501(c)(3) | 1946 | | | |
| St Johns | | 501(c)(3) | 1988 | | | 2013-12 |
| Stutsman County 4-H | Youth development | 501(c)(3) | 2025 | $0 | $0 | 2025-08 |
| Stutsman County Fair Foundation | Food, agriculture and nutrition | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| Stutsman County Farm Bureau | Food, agriculture and nutrition | 501(c)(5) | 1977 | $0 | $0 | 2025-12 |
| Stutsman County Human Rights Coalition | Arts, culture and humanities | 501(c)(3) | 2021 | $0 | $0 | 2025-12 |
| Stutsman County Memorial Museum | Arts, culture and humanities | 501(c)(3) | 2006 | $0 | $0 | 2025-10 |
| T & K Kids First | Health care | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Temple Baptist Church of Jamestown | | 501(c)(3) | 1972 | | | |
| Thomas Avery Doyle Memorial Fund | Mental health and crisis intervention | 501(c)(3) | 2016 | $0 | $0 | 2025-09 |
| Tops Club Inc | Health care | 501(c)(3) | 2008 | $0 | $0 | 2026-03 |
| Unite To Silence the Stigma - Serving the Jamestown Area | Mental health and crisis intervention | 501(c)(3) | 2016 | $0 | $0 | 2023-12 |
| Urdahl Inc | | 501(c)(3) | 1992 | | $403,992 | 2026-03 |
| Washington Elementary Pto | Education | 501(c)(3) | 2020 | $0 | $0 | 2025-07 |
| William S Gussner Elementary Parent Teacher Organization | Education | 501(c)(3) | 2014 | $0 | $0 | 2025-06 |
| Wings On Strings | Recreation and sports | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| Zonta International | | 501(c)(4) | 1953 | $0 | $0 | 2025-05 |