| Orlando Health Inc | 501(c)(3) | 1977 | $7.30 billion | $11.09 billion | 2025-09 |
| Orlando Health Medical Group Inc | 501(c)(3) | 1997 | $1.25 billion | $449.1 million | 2025-09 |
| Oneblood Inc | 501(c)(3) | 1994 | $450.7 million | $491.6 million | 2024-12 |
| Assistance Fund Inc | 501(c)(3) | 2009 | $284.7 million | $565.6 million | 2025-12 |
| Orlando Health Foundation Inc | 501(c)(3) | 1983 | $69.7 million | $270.2 million | 2025-09 |
| Positive Assistance | 501(c)(3) | 2015 | $14.1 million | $3.7 million | 2024-12 |
| Guardian Care Inc | 501(c)(3) | 1971 | $10.6 million | $20.7 million | 2024-06 |
| Power To the Patients | 501(c)(3) | 2023 | $9.6 million | $1.5 million | 2024-12 |
| Crystal Lake Supportive Environments Inc | 501(c)(3) | 1990 | $9.5 million | $6.5 million | 2025-12 |
| Pineapple Healthcare | 501(c)(3) | 2020 | $9.4 million | $3.9 million | 2024-12 |
| Two-6 Resources Inc | 501(c)(3) | 2006 | $8.9 million | $8.9 million | 2025-12 |
| MHP Salud | 501(c)(3) | 1994 | $6.6 million | $1.6 million | 2025-12 |
| Ronald McDonald House Central Florida Inc | 501(c)(3) | 1996 | $6.2 million | $17.5 million | 2025-12 |
| HCCH Holding Corporation | 501(c)(3) | 2019 | $5.9 million | $5.2 million | 2025-09 |
| Grace Medical Home Inc | 501(c)(3) | 2009 | $5.4 million | $3 million | 2024-12 |
| Crew Community Health | 501(c)(3) | 2020 | $4.3 million | $3 million | 2025-12 |
| Embrace Health | 501(c)(3) | 2022 | $3.4 million | $880,172 | 2025-12 |
| Lake Nona Institute Inc | 501(c)(3) | 2011 | $2.1 million | $2.4 million | 2025-12 |
| Spektrum Health | 501(c)(3) | 2018 | $1.9 million | $151,800 | 2025-06 |
| Mothers Milk Bank of Florida | 501(c)(3) | 2012 | $1.9 million | $1.5 million | 2024-12 |
| Florida Nurses Association Inc | 501(c)(6) | 1945 | $1.6 million | $1.8 million | 2024-12 |
| Ucf Academic Health Inc | 501(c)(3) | 2019 | $1.4 million | $94.6 million | 2025-06 |
| Bridge To Independence Inc | 501(c)(3) | 2003 | $1.2 million | $3.2 million | 2025-12 |
| Primary Care Access Network Inc | 501(c)(3) | 2015 | $837,943 | $442,642 | 2025-12 |
| Wound Care Collaborative Community Inc | 501(c)(3) | 2023 | $598,385 | $476,196 | 2025-12 |
| Helping Oppressed & Marginalized Communities To Elevate Inc | 501(c)(3) | 2020 | $594,536 | $1.3 million | 2024-12 |
| Benjamin Price Charitable Trust | 501(c)(3) | 2020 | $441,355 | $3.6 million | 2025-12 |
| Oic Inspired | 501(c)(3) | 2025 | $409,684 | $51,881 | 2025-12 |
| Health Care 4 All Inc | 501(c)(3) | 2023 | $354,531 | $378,624 | 2025-06 |
| Invested Health Center Inc | 501(c)(3) | 2023 | $324,773 | $120,198 | 2025-12 |
| Got Ur 6 Inc | 501(c)(3) | 2020 | $282,590 | $22,465 | 2025-12 |
| Triple C Foundation Inc | 501(c)(3) | 2022 | $174,915 | $378,659 | 2025-12 |
| Aguilar Salud Inc | 501(c)(3) | 2023 | $166,913 | $12,023 | 2025-12 |
| Dental Care Access Foundation Inc | 501(c)(3) | 2005 | $140,900 | $76,454 | 2025-12 |
| GMH Property Holdings Inc | 501(c)(3) | 2017 | $133,952 | $5.6 million | 2024-12 |
| Central Florida Softball League Inc | 501(c)(3) | 2014 | $131,963 | $71,365 | 2024-12 |
| Not A Dry Eye Foundation Inc | 501(c)(3) | 2015 | $128,858 | $43,069 | 2025-12 |
| Friends of Fisher House Orlando | 501(c)(3) | 2016 | $119,153 | $657,404 | 2025-12 |
| Healing the Children-Florida Inc | 501(c)(3) | 1998 | $117,203 | $435,819 | 2024-12 |
| Health and Education for Less Privileged Inc | 501(c)(3) | 2020 | $113,077 | $22,659 | 2025-12 |
| Variety-The Childrens Charity of Florida Inc | 501(c)(3) | 1991 | $105,801 | $60,432 | 2025-09 |
| D B Peru Inc | 501(c)(3) | 2003 | $84,788 | $3,433 | 2025-12 |
| Accessible Acupuncture Initiative Inc | 501(c)(3) | 2016 | $66,547 | $6,519 | 2025-12 |
| The American Board of Urgent Care Medicine Inc | 501(c)(6) | 2000 | $65,770 | $64,722 | 2025-12 |
| National Early Childhood Cancer Foundation Inc | 501(c)(3) | 2010 | $52,394 | $82 | 2024-12 |
| Dream Cars 4 Kids East | 501(c)(3) | 2024 | $46,885 | $3,589 | 2025-12 |
| Harwell Family Foundation Inc | 501(c)(3) | 2018 | $39,756 | $1 million | 2024-12 |
| Coamed Foundation Inc | 501(c)(3) | 2015 | $36,265 | $54,329 | 2024-12 |
| Benji Watson Cancer Foundation Inc | 501(c)(3) | 2011 | $18,117 | $18,898 | 2025-09 |
| Crossway Kidney Care Foundation Inc | 501(c)(3) | 2018 | $13,560 | $1 | 2025-12 |
| Galen Foundation Inc | 501(c)(3) | 2022 | $9,346 | $128,023 | 2025-12 |
| Nigerian Nurses Association Florida Inc | 501(c)(3) | 2023 | $3,230 | $61,579 | 2025-12 |
| Acupuncture Fit Initiative Inc | 501(c)(3) | 2023 | $570 | $119 | 2025-12 |
| Shades Hopes Dreams Inc | 501(c)(3) | 2012 | $510 | $3,116 | 2025-12 |
| A Heart for Moms Inc | 501(c)(3) | 2026 | $0 | $0 | 2025-12 |
| Ahwa Foundation Inc | 501(c)(3) | 2010 | $0 | $5,626 | 2024-12 |
| Ajh Core Care Corporation | 501(c)(3) | 2023 | $0 | $0 | 2025-12 |
| All Empowered Foundation | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| American Radon Network Inc | 501(c)(3) | 2026 | | | |
| Association for Professionals in Infection CTRL & Epidemiology Inc | 501(c)(3) | 1975 | $0 | $0 | 2025-12 |
| Association of Clinical Research Professionals | 501(c)(3) | 1984 | $0 | $0 | 2024-12 |
| Baby Mine Project Inc | 501(c)(3) | 2019 | $0 | $0 | 2025-12 |
| Bacon Foundation Inc | 501(c)(3) | 2014 | $0 | $0 | 2024-12 |
| Believen Organization Inc | 501(c)(3) | 2024 | $0 | $0 | 2026-04 |
| Brain Spine and Pain Foundation Inc | 501(c)(3) | 2025 | | | |
| Bridge Community Connections Inc | 501(c)(3) | 2018 | $0 | $0 | 2025-12 |
| CMJ Resources & Development Group Inc | 501(c)(3) | 2023 | $0 | $0 | 2024-12 |
| Central Florida Society of Hospital Pharmacists Inc | 501(c)(6) | 2009 | $0 | $0 | 2025-12 |
| Clearpath Chiropractic | 501(c)(3) | 2019 | $0 | $0 | 2025-12 |
| Clerjo Community Outreach Foundatio N Inc | 501(c)(3) | 2026 | | | |
| Collateral Pathway Foundation Incorporated | 501(c)(3) | 2025 | | | |
| Elizabeth Scovil Heart Foundation Inc | 501(c)(3) | 2018 | $0 | $0 | 2025-12 |
| Equal All Initiative Inc | 501(c)(3) | 2025 | $0 | $0 | 2025-03 |
| Ethan Garza Foundation | 501(c)(3) | 2025 | | | |
| First Embrace Incorporated | 501(c)(3) | 2025 | | | |
| Five Pillars Free Clinic Inc | 501(c)(3) | 2020 | $0 | $0 | 2025-12 |
| Florida Integrative Rehab Inc | 501(c)(3) | 2015 | $0 | $0 | 2024-12 |
| Franco Medical Care Corp | 501(c)(3) | 2026 | | | |
| Gifting of A Lifetime Experience Inc | 501(c)(3) | 2023 | $0 | $0 | 2023-12 |
| Global Wellness & Care Relief Foundation | 501(c)(3) | 2026 | | | |
| Glow and Grow Foundation Incorporated | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| Harmony Healthcare Orlando Inc | 501(c)(3) | 2019 | | $6 million | 2024-12 |
| Health Centers United Inc | 501(c)(3) | 2011 | $0 | $0 | 2025-12 |
| Healthcare Access Alliance | 501(c)(3) | 2018 | $0 | $0 | 2024-12 |
| Healthcare Professionals Wellness Association Inc | 501(c)(3) | 2025 | | | |
| Help Haven | 501(c)(3) | 2023 | $0 | $0 | 2023-12 |
| International Birth Center Incorpor Ated | 501(c)(3) | 2025 | | | |
| International Topical Steroid Withdrawal Organization | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| International Tracheoesophageal Fistula Foundation Inc | 501(c)(3) | 2026 | | | |
| Jay Khodiyar & Jay Maharaj Health Services Inc | 501(c)(3) | 2019 | $0 | $0 | 2024-12 |
| John Paul Charitable Foundation Inc | 501(c)(3) | 2025 | | $5,000 | 2025-12 |
| Joyful Music Therapy Foundation Inc | 501(c)(3) | 2017 | $0 | $0 | 2025-12 |
| Lauren Boss Legacy Foundation | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Life Beyond Parkinsons Corp | 501(c)(3) | 2026 | | | |
| Life Wise Choices Health Coalition Inc | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Lighting the Darkness Inc | 501(c)(3) | 2019 | $0 | $0 | 2025-12 |
| Medical Missions Saving Lives Corporation | 501(c)(3) | 2021 | $0 | $0 | 2025-12 |
| Medical and Education Foundation Inc | 501(c)(3) | 2016 | $0 | $0 | 2023-06 |
| Mentor Institute Inc | 501(c)(3) | 2025 | | | |
| Michals Hope Inc | 501(c)(3) | 2023 | $0 | $0 | 2025-12 |
| National Association of Hispanic Nurses | 501(c)(3) | 2005 | $0 | $0 | 2025-12 |
| Nurses Deserve Inc | 501(c)(3) | 2025 | | | |
| Ohi Lakeland Highlands Inc | 501(c)(3) | 2025 | $0 | $1 | 2025-09 |
| Ohi Wiregrass Ranch Inc | 501(c)(3) | 2025 | $0 | $1 | 2025-09 |
| On the Wings of Love Outreach Inc | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| Pacific Health Partners Inc | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Paradise Care Center Inc | 501(c)(3) | 2023 | $0 | $1 | 2025-12 |
| Phreedom Foundation Inc | 501(c)(3) | 2023 | $0 | $0 | 2024-12 |
| Pink Ribbon Princess Inc | 501(c)(3) | 2012 | $0 | $0 | 2025-12 |
| Project Behavior Change | 501(c)(3) | 2024 | | | |
| Reaching New Horizons for Autism | 501(c)(3) | 2014 | $0 | $0 | 2025-12 |
| Reaching Smiles Foundation | 501(c)(3) | 2023 | $0 | $0 | 2025-12 |
| Redefining Spina Bifida Inc | 501(c)(3) | 2015 | $0 | $0 | 2025-12 |
| Resurgence Health Inc | 501(c)(3) | 2023 | $0 | $0 | 2025-06 |
| Ritah Foundation Inc | 501(c)(3) | 2019 | $0 | $444 | 2023-12 |
| Sakhuja Family Foundation Inc | 501(c)(3) | 2018 | | $488,261 | 2024-12 |
| Savepeptides Inc | 501(c)(3) | 2020 | $0 | $0 | 2025-12 |
| Scuba 4 Life Inc | 501(c)(3) | 2020 | $0 | $0 | 2025-12 |
| Seed Wellness | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| Senior Movements 4 Life Inc | 501(c)(3) | 2020 | $0 | $0 | 2025-12 |
| Sharing Smiles International Inc | 501(c)(3) | 2026 | $0 | $0 | 2025-12 |
| Specialcare of Central Florida Inc | 501(c)(3) | 2007 | $0 | $0 | 2025-09 |
| Stanced On Paralysis Inc | 501(c)(3) | 2019 | $0 | $0 | 2024-01 |
| Stellar Healing Center Inc | 501(c)(3) | 2026 | | | |
| Sunshine State Dental Aid Inc | 501(c)(3) | 2024 | | | |
| Teens Thriving Together Inc | 501(c)(3) | 2022 | $0 | $0 | 2025-12 |
| The Male Wellness Collective Inc | 501(c)(3) | 2015 | $0 | $0 | 2025-12 |
| The Smile Movement Inc | 501(c)(3) | 2026 | | | |
| Thenever Alone Nick Rucker Foundation Inc | 501(c)(3) | 2022 | $0 | $0 | 2025-12 |
| Tops Club Inc | 501(c)(3) | 2008 | $0 | $0 | 2026-03 |
| Tortuga Island United-Org | 501(c)(3) | 2024 | | $107,404 | 2024-12 |
| Transfusion Medicine Specialists Inc | 501(c)(3) | 1999 | $0 | $0 | 2025-12 |
| Type Zero Foundation Inc | 501(c)(3) | 2016 | $0 | $0 | 2025-07 |
| United Futures Foundation Corp | 501(c)(3) | 2026 | | | |
| Valiant Ridge Foundation | 501(c)(3) | 2026 | | | |
| Vip Health Foundation Corp | 501(c)(2) | 2026 | | | |
| Vision Heritage | 501(c)(3) | 2008 | $0 | $0 | 2025-12 |
| Visiting Nurse Association of Central Florida Inc | 501(c)(3) | 1984 | $0 | $0 | 2024-09 |
| We Are One Medical Group Inc | 501(c)(3) | 2017 | $0 | $0 | 2023-12 |
| We Push Health Foundation Inc | 501(c)(3) | 2024 | $0 | $0 | 2024-12 |
| With Ties of Love Inc | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| Women Surgical Services Inc | 501(c)(3) | 2021 | $0 | $0 | 2024-12 |