| Florida Southern College | 501(c)(3) | 1941 | $188.2 million | $359.3 million | 2025-05 |
| Southeastern University Inc | 501(c)(3) | 1947 | $158.8 million | $159.1 million | 2025-05 |
| Polk County School Readiness Coalition Inc | 501(c)(3) | 2000 | $58.7 million | $6.8 million | 2025-06 |
| The Schools of McKeel Academy Inc | 501(c)(3) | 1999 | $36.7 million | $43 million | 2025-06 |
| Lakeland Christian School Inc | 501(c)(3) | | $25.7 million | $69.6 million | 2025-06 |
| New Beginnings High School | 501(c)(3) | 2011 | $11.1 million | $6.3 million | 2025-06 |
| Achievement Academy Inc | 501(c)(3) | 1957 | $4.3 million | $3.8 million | 2025-06 |
| Academy Prep Foundation Inc | 501(c)(3) | 1997 | $3.8 million | $44.3 million | 2025-05 |
| Mi Escuela Montessori Inc | 501(c)(3) | 2019 | $3.5 million | $1.5 million | 2025-06 |
| Geneva Classical Academy Inc | 501(c)(3) | 1997 | $3 million | $3.8 million | 2025-06 |
| Academy Prep Center of Lakeland Inc | 501(c)(3) | 2018 | $3 million | $507,025 | 2025-05 |
| Aerospace Center for Excellence Inc | 501(c)(3) | 2001 | $2.5 million | $10.8 million | 2025-06 |
| Magnolia Montessori Academy Inc | 501(c)(3) | 2013 | $2.3 million | $2.9 million | 2025-06 |
| Lakeland Montessori Schoolhouse Inc | 501(c)(3) | 2004 | $2.1 million | $6.1 million | 2025-06 |
| Florida Polytechnic University Foundation Inc | 501(c)(3) | 2013 | $2.1 million | $13.6 million | 2025-06 |
| Dream Center of Lakeland Inc | 501(c)(3) | 2003 | $2 million | $1.5 million | 2024-12 |
| Smart Start Youth & Family Services Inc | 501(c)(3) | 2007 | $1.2 million | $257,192 | 2026-06 |
| Sparrow Education Foundation Inc | 501(c)(3) | 2020 | $1.2 million | $70,880 | 2024-12 |
| Lakeland Institute for Learning Org | 501(c)(3) | 2015 | $1.2 million | $634,985 | 2025-06 |
| Learning Resource Center of Polk Co | 501(c)(3) | 1977 | $1.1 million | $780,146 | 2024-06 |
| Florida School of Preaching | 501(c)(3) | 1974 | $718,288 | $1.9 million | 2025-06 |
| Central Florida Aerospace Academy Foundation Inc | 501(c)(3) | 2009 | $528,909 | $2.4 million | 2025-06 |
| Wilson House Inc | 501(c)(3) | 1994 | $360,987 | $198,979 | 2025-12 |
| Florida Council On Crime and Delinquency Inc | 501(c)(3) | 2019 | $309,547 | $236,406 | 2025-09 |
| Global Health Impact Inc | 501(c)(3) | 2024 | $233,335 | $81,190 | 2025-12 |
| Linking Community Now Inc | 501(c)(3) | 2015 | $221,985 | $39,232 | 2024-12 |
| Wonderhere Education Foundation | 501(c)(3) | 2020 | $152,478 | $75,871 | 2024-12 |
| Lakeland Senior High School Boosters Club Inc | 501(c)(3) | 1965 | $139,469 | $227,792 | 2025-06 |
| Platform Art Inc | 501(c)(3) | 2006 | $138,780 | $90,763 | 2024-12 |
| Inspiration Academy of Lakeland Inc | 501(c)(3) | 2024 | $135,000 | $1,923 | 2024-12 |
| Dreadnaught Band Boosters Inc | 501(c)(3) | 1988 | $125,086 | $20,211 | 2023-06 |
| National Tutoring Association | 501(c)(3) | 2005 | $119,983 | $122,188 | 2025-12 |
| Read Lakeland Inc | 501(c)(3) | 1996 | $93,216 | $78,652 | 2026-03 |
| Lakeland Senior High School Dugoutclub Inc | 501(c)(3) | 2006 | $71,297 | $136,053 | 2025-06 |
| Eagle Club Inc | 501(c)(3) | 2012 | $63,722 | $11,388 | 2025-12 |
| Polk County Medical Foundation | 501(c)(3) | 2002 | $62,383 | $286,835 | 2024-12 |
| Lincoln Avenue Academy Pto Inc | 501(c)(3) | 2026 | $49,246 | $91,176 | 2025-06 |
| Washington Park-Rochelle High Schools Alumni Association | 501(c)(3) | 1995 | $38,167 | $152,437 | 2025-10 |
| Lawton Chiles Middle Academy Ptso Inc | 501(c)(3) | 2018 | $23,716 | $31,571 | 2025-06 |
| Cleveland Heights 4-Ball Invitational Inc | 501(c)(3) | 2005 | $15,779 | $12,207 | 2025-12 |
| 1600 Academic Rewards Inc | 501(c)(3) | 2024 | $0 | $0 | 2024-12 |
| Against All Odds Ministry Inc | 501(c)(3) | 2017 | $0 | $0 | 2025-12 |
| Against All Odds Scholarship Foundation Inc | 501(c)(3) | 2012 | $0 | $0 | 2025-12 |
| Arzillo Ministries Inc | 501(c)(3) | 2023 | $0 | $0 | 2025-12 |
| Association for Marketing and Healthcare Research Inc | 501(c)(3) | 2022 | $0 | $0 | 2025-12 |
| Auburndale Njrotc Booster Club | 501(c)(3) | 2023 | | $1,490 | 2026-06 |
| BB Tutoring Foundation Inc | 501(c)(3) | 2026 | | | |
| Beacon Legacy Foundation | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| Benjamin Franklin Academy | 501(c)(3) | 2019 | $0 | $0 | 2025-12 |
| Betsye Kay Finch Performing Arts Foundation Inc | 501(c)(3) | 2019 | $0 | $0 | 2025-12 |
| Blake-Davis Medical Academy | 501(c)(3) | 2024 | | | |
| Booking It Back To School Inc | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| Breadandbooks Org Inc | 501(c)(3) | 2018 | $0 | $0 | 2023-12 |
| Bright Imaginations Preparatory Schools Inc | 501(c)(3) | 2025 | $0 | $0 | 2025-06 |
| Buffalo Soldiers Florida | 501(c)(3) | 2019 | $0 | $0 | 2025-05 |
| Caf Christian Academy Inc | 501(c)(3) | 2022 | $0 | $0 | 2025-12 |
| Campisi Family Foundation Inc | 501(c)(3) | 2014 | | $6.8 million | 2025-12 |
| Career Glimpse LLC | 501(c)(3) | 2023 | $0 | $0 | 2023-12 |
| Cleveland Court Elementary Pto Inc | 501(c)(3) | 2007 | $0 | $0 | 2025-06 |
| Clint Wright Foundation Inc | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Dancehall for A Cause Corporation | 501(c)(3) | 2021 | $0 | $0 | 2025-12 |
| Dauphin Street Community and Educational Center Inc | 501(c)(3) | 2022 | $0 | $0 | 2023-12 |
| Double M Foundation | 501(c)(3) | 2020 | $0 | $0 | 2025-12 |
| Driven With A Purpose Academy Inc | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| Emily Hughes Foundation | 501(c)(3) | 2026 | | | |
| Falls Adventure Inc | 501(c)(3) | 1996 | $0 | $0 | 2024-12 |
| Florida Theological Seminary and Bible College Inc | 501(c)(3) | 2009 | | | |
| Floridian Future Inc | 501(c)(4) | 2026 | | | |
| Gamma Delta Chapter of Theta Chi | 501(c)(7) | 2015 | $0 | $0 | 2026-06 |
| Goal Seekers and Eagles of Lakeland Inc | 501(c)(3) | 2015 | | $61,543 | 2025-02 |
| Goodman Academy of Possibilities Inc | 501(c)(3) | 2020 | $0 | $0 | 2025-12 |
| Harrison Alumni Relations Committee | 501(c)(3) | 2020 | $0 | $0 | 2026-05 |
| Healing United | 501(c)(3) | 2020 | $0 | $0 | 2025-12 |
| Highlands Grove Elementary Pto | 501(c)(3) | 2014 | $0 | $0 | 2026-07 |
| Home Oriented Parent Educators of Central Florida Inc | 501(c)(3) | 2019 | $0 | $0 | 2026-06 |
| Honor Society of Phi Kappa Phi | 501(c)(3) | 1999 | $0 | $0 | 2025-06 |
| I Rise I Succeed Center for Excellence | 501(c)(3) | 2022 | $0 | $0 | 2025-12 |
| Imperial Polk Genealogical Society Inc | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Integrity Arts & Education Inc | 501(c)(3) | 2025 | | | |
| Interact Council for Human Development Inc | 501(c)(3) | 1997 | | | |
| Joyous Financial Wellness Inc | 501(c)(3) | 2026 | $0 | $0 | 2024-12 |
| Just 4 U Educational Solutions Inc | 501(c)(3) | 2023 | $0 | $0 | 2023-12 |
| Kelsey Higgenbotham Family Community Scholarship Fund Trust | 501(c)(3) | 2009 | $0 | $0 | 2025-12 |
| Kids Need Both Inc | 501(c)(3) | 2013 | $0 | $0 | 2026-01 |
| Lakeland Ffa Alumni | 501(c)(3) | 2023 | $0 | $0 | 2024-07 |
| Lakeland Highlands Middle School Ptso Inc | 501(c)(3) | 2010 | $0 | $0 | 2025-06 |
| Lambda Chi Alpha Fraternity Inc | 501(c)(7) | 2025 | $0 | $0 | 2025-12 |
| McKeel Academy of Technology Ptso Inc | 501(c)(3) | 2017 | $0 | $0 | 2026-06 |
| McKeel Elementary Academy Pto Inc | 501(c)(3) | 2006 | $0 | $0 | 2025-05 |
| Media Education Society Inc | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Montessori Middle School Inc | 501(c)(3) | 2010 | $0 | $0 | 2025-06 |
| Nbe Specialty Consulting | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| Nooktoria Legal Resource | 501(c)(3) | 2026 | | | |
| Ovarian Cancer Support Group of Polk County Florida Inc | 501(c)(3) | 2016 | $0 | $0 | 2025-12 |
| Parent Booster USA Inc | 501(c)(3) | 2005 | $0 | $0 | 2025-06 |
| Phi Eta Sigma National Honor Society Inc | 501(c)(3) | 2014 | | | |
| Philip O Brien Elementary Pto | 501(c)(3) | 2026 | | | |
| Planes | 501(c)(3) | 2023 | $0 | $0 | 2025-06 |
| Polk County Retired Educators Association Polk 1 Inc | 501(c)(3) | 2023 | $0 | $0 | 2026-06 |
| Polk Microschool Collective | 501(c)(3) | 2026 | | | |
| Polk Scholastic Chess Inc | 501(c)(3) | 2005 | $0 | $0 | 2025-07 |
| Project Inspiration | 501(c)(3) | 2015 | $0 | $0 | 2025-12 |
| Reading With Erma Inc | 501(c)(3) | 2019 | $0 | $0 | 2025-12 |
| Ridge Beekeepers Association Inc | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Scott Lake Elementary Pto | 501(c)(3) | 2014 | $0 | $0 | 2025-06 |
| She Ascends Inc | 501(c)(3) | 2024 | | | |
| Sigma Theta Tau International Inc | 501(c)(3) | 1954 | $0 | $0 | 2023-06 |
| Skilled Hearts | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| South McKeel Elementary Academy Pto Inc | 501(c)(3) | 2009 | $0 | $0 | 2025-06 |
| Southeastern University Foundation Inc | 501(c)(3) | 2014 | $0 | $1 | 2025-05 |
| Sun N Fun Chapter 454 Eaa Inc | 501(c)(3) | 2019 | $0 | $0 | 2025-12 |
| Teachers of the Year National Alum | 501(c)(3) | 2006 | $0 | $0 | 2024-12 |
| Teens Educators Moms Pops Organization Tempo of Lakeland | 501(c)(3) | 1992 | $0 | $0 | 2025-07 |
| Tenoroc Ffa Alumni | 501(c)(3) | 2015 | $0 | $0 | 2026-06 |
| The Budgeting and Understanding Finances Foundation | 501(c)(3) | 2026 | | | |
| The Class of 89 Alumni Incorporated | 501(c)(3) | 2026 | | | |
| The Serena Bailey Foundation Inc | 501(c)(3) | 2004 | | $875,109 | 2025-12 |
| Upsilon Xi Chapter of Omega Psi Phi Fraternity Inc | 501(c)(7) | 2024 | | | |
| We Are Now | 501(c)(3) | 2019 | $0 | $0 | 2025-12 |
| Wild Dandelions Services Inc | 501(c)(3) | 2024 | | | |
| Wonder Boxed Stem Education Inc | 501(c)(3) | 2025 | | | |