| Southern Baptist Hospital of Florida Inc | 501(c)(3) | 1971 | $2.97 billion | $5.18 billion | 2025-09 |
| The Nemours Foundation | 501(c)(3) | 1981 | $2.36 billion | $3.43 billion | 2024-12 |
| Shands Jacksonville Medical Center Inc | 501(c)(3) | 1982 | $1.33 billion | $1.26 billion | 2025-06 |
| University of Florida Jacksonville Physicians Inc | 501(c)(3) | 1979 | $520.5 million | $192.6 million | 2025-06 |
| Alfred I Dupont Charitable Trust | 501(c)(3) | 2018 | $498.8 million | $8.77 billion | 2024-12 |
| Baptist Health System Inc | 501(c)(3) | 1985 | $289.1 million | $852.9 million | 2025-09 |
| Baptist Medical Center Beaches | 501(c)(3) | 1990 | $243.5 million | $329.6 million | 2025-09 |
| Brooks Rehabilitation Hospital | 501(c)(3) | 1995 | $192.8 million | $192.1 million | 2024-12 |
| Baptist Medical Center of Nassau I NC | 501(c)(3) | 1994 | $146.6 million | $179.3 million | 2025-09 |
| Genesis Health Inc | 501(c)(3) | 1983 | $56.4 million | $634.7 million | 2024-12 |
| Florida Proton Therapy Institute Inc | 501(c)(3) | 2002 | $53.1 million | $141.3 million | 2025-06 |
| Mental Health Resource Center Inc | 501(c)(3) | 1980 | $45.9 million | $23.8 million | 2025-06 |
| Brooks Skilled Nursing Facility A Inc | 501(c)(3) | 2011 | $37.7 million | $10.6 million | 2024-12 |
| Brooks Home Care Advantage | 501(c)(3) | 2009 | $37.3 million | $16.9 million | 2024-12 |
| Pediatric Medical Services of Florida Inc | 501(c)(3) | 2012 | $31 million | $9.4 million | 2024-12 |
| River Garden Hebrew Home for the Aged | 501(c)(3) | 1948 | $27 million | $16.4 million | 2024-12 |
| Fannie E Taylor Home for the Aged Care Center Inc | 501(c)(3) | 1983 | $16 million | $1 million | 2025-12 |
| Florida Senior Housing Initiative Inc | 501(c)(3) | 2024 | $14.3 million | $516,734 | 2024-12 |
| Ability Housing Inc | 501(c)(3) | 1992 | $12.1 million | $44.3 million | 2025-12 |
| Florida Association of Free and Charitable Clinics Inc | 501(c)(3) | 2013 | $12.1 million | $1.8 million | 2025-12 |
| Physical Medicine Specialists Inc | 501(c)(3) | 1998 | $11.8 million | $3.5 million | 2024-12 |
| Muslim American Social Services Inc | 501(c)(3) | 2014 | $10.3 million | $838,003 | 2025-12 |
| Agape Community Health Center Inc | 501(c)(3) | 2016 | $9.7 million | $10.4 million | 2025-12 |
| Cathedral Gerontology Center Inc | 501(c)(3) | 2000 | $9 million | $2.1 million | 2025-09 |
| Community Hospice of Northeast Florida Foundation for Caring Inc | 501(c)(3) | 2000 | $6.9 million | $31.7 million | 2025-09 |
| Managed Access To Child Health Inc | 501(c)(3) | 1994 | $6.8 million | $4.3 million | 2025-06 |
| Renaissance Behavioral Health Systems Inc | 501(c)(3) | 1990 | $5.3 million | $6.2 million | 2025-06 |
| HB Rehabilitative Services Inc | 501(c)(3) | 2013 | $5.1 million | $11 million | 2024-12 |
| Coastal Community Health Inc | 501(c)(3) | 2015 | $4.3 million | $457,419 | 2025-09 |
| Health Planning Council of Northeast Florida Inc | 501(c)(3) | 1983 | $4.2 million | $1.5 million | 2025-06 |
| Baptist Health Ambulatory Services Inc | 501(c)(3) | 1997 | $4.2 million | $4.3 million | 2025-09 |
| First Coast Womens Services | 501(c)(3) | 1994 | $4 million | $7.3 million | 2025-12 |
| Ronald McDonald House Jacksonville Inc | 501(c)(3) | 1986 | $3.9 million | $25.3 million | 2025-12 |
| Volunteers in Medicine Jacksonville Inc | 501(c)(3) | 2002 | $3.2 million | $2.7 million | 2025-09 |
| Jacksonville Speech and Hearing Center Inc | 501(c)(3) | 1964 | $2.5 million | $1.7 million | 2025-06 |
| Alivia Care Solutions | 501(c)(3) | 2022 | $2.4 million | $1.1 million | 2025-09 |
| We Care Jacksonville Inc | 501(c)(3) | 1997 | $2 million | $786,066 | 2025-09 |
| Northeast Florida Area Health | 501(c)(3) | 1994 | $1.6 million | $1 million | 2025-06 |
| V for Victory Over Cancer | 501(c)(3) | 2017 | $1.5 million | $982,740 | 2024-12 |
| Genesis Health Foundation Inc | 501(c)(3) | 1983 | $1.5 million | $8.5 million | 2024-12 |
| Vision Is Priceless Council Inc | 501(c)(3) | 1996 | $962,190 | $2.8 million | 2025-06 |
| Brooks Skilled Nursing Facility Holdings B Inc | 501(c)(3) | 2013 | $873,542 | $19.9 million | 2024-12 |
| HB Outpatient Rehabilitative Services Inc | 501(c)(3) | 2019 | $822,452 | $2.5 million | 2024-12 |
| Northeast Florida Healthcare Coalition Inc | 501(c)(3) | 2017 | $748,884 | $0 | 2025-06 |
| Strive Youth and Family Services Inc | 501(c)(3) | 2023 | $743,267 | $222,199 | 2025-12 |
| Womens Help Center Inc | 501(c)(3) | 1992 | $725,245 | $2.2 million | 2024-12 |
| St Johns River Rual Health Network Inc | 501(c)(3) | 1996 | $592,258 | $511,241 | 2025-09 |
| The Nest A Womens Center Inc | 501(c)(3) | 2016 | $488,732 | $611,050 | 2025-12 |
| Angel Kids Foundation Inc | 501(c)(3) | 2019 | $440,652 | $381,907 | 2024-12 |
| Center for Health and Development Initiative Inc | 501(c)(3) | 2025 | $348,551 | $324,964 | 2025-12 |
| Mayfair Womens Clinic Inc | 501(c)(3) | 2022 | $261,061 | $160,110 | 2024-12 |
| Sci Functional Health and Nutrition Inc | 501(c)(3) | 2024 | $211,390 | $120,000 | 2025-12 |
| Knights of Rizal of Greater Jacksonville Inc | 501(c)(3) | 1991 | $203,079 | $1.2 million | 2025-12 |
| Healing Heart Project | 501(c)(3) | 2017 | $174,512 | $330,789 | 2024-12 |
| Gods House International Christian Fellowship | 501(c)(3) | 2018 | $165,735 | $13,690 | 2024-12 |
| BGC Foundation for Research and Education Inc | 501(c)(3) | 2008 | $163,891 | $565,994 | 2024-12 |
| Patric Young Foundation Inc | 501(c)(3) | 2023 | $121,588 | $124,564 | 2024-12 |
| Care Across Continents Inc | 501(c)(3) | 2025 | $118,533 | $89,174 | 2025-12 |
| Horse Sense & Sensitivity Inc | 501(c)(3) | 2005 | $97,255 | $63,604 | 2025-12 |
| Apel Health Services Center Inc | 501(c)(3) | 2005 | $89,616 | $1 | 2025-12 |
| First Coast Worksite Wellness Council Inc | 501(c)(3) | 2010 | $89,081 | $101,746 | 2026-06 |
| American Board of Clinical Lipidology Inc | 501(c)(3) | 2005 | $88,315 | $163,440 | 2025-12 |
| Academy On Violence and Abuse | 501(c)(3) | 2005 | $82,185 | $171,765 | 2025-12 |
| Arlington Lions Foundation Inc | 501(c)(3) | 1996 | $78,237 | $3.2 million | 2025-06 |
| Lifeline Resources Inc | 501(c)(3) | 2010 | $77,459 | $109,426 | 2025-12 |
| Wave of Support Inc | 501(c)(3) | 2025 | $75,500 | $24,246 | 2025-12 |
| Chemo Noir Inc | 501(c)(3) | 2017 | $57,277 | $21,972 | 2024-12 |
| Methodist Medical Center Inc | 501(c)(3) | 1967 | $40,941 | $57.9 million | 2025-06 |
| Association of Fatima Jinnah Old Graduates Inc | 501(c)(3) | 2019 | $35,000 | $52,235 | 2025-06 |
| Center of Achievement Inc | 501(c)(3) | 2009 | $15,124 | $76,604 | 2024-12 |
| Home Call Healthcare Corp | 501(c)(3) | 2015 | $10,779 | $0 | 2024-12 |
| Legacy of Care Health Center Inc | 501(c)(3) | 2008 | $9,825 | $18,590 | 2025-12 |
| A Guiding Light | 501(c)(3) | 2019 | $0 | $0 | 2025-12 |
| Adonais Care | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Adore Me Nanny | 501(c)(3) | 2023 | $0 | $0 | 2024-12 |
| Affinity Outreach Inc | 501(c)(3) | 2025 | | | |
| Arlington Eye Clinic | 501(c)(3) | 2015 | | $60,560 | 2025-06 |
| Association for Professionals in Infection CTRL & Epidemiology Inc | 501(c)(3) | 1975 | $0 | $0 | 2025-12 |
| Association of Clinical Research Professionals | 501(c)(3) | 1984 | $0 | $0 | 2024-12 |
| Association of Nurses in Aids Care | 501(c)(3) | 2004 | | | |
| Attainable Care Inc | 501(c)(3) | 2025 | | | |
| Bikur Cholim of Northeast Florida Inc | 501(c)(3) | 2023 | $0 | $0 | 2025-12 |
| Brooks Skilled Nursing Inc | 501(c)(3) | 2010 | $0 | $0 | 2024-12 |
| Cardiovision Care Foundation | 501(c)(3) | 2026 | | | |
| Chores Inc | 501(c)(3) | 2000 | $0 | $0 | 2025-12 |
| Closest To Home Inc | 501(c)(3) | 2026 | $0 | $0 | 2025-12 |
| Core Life Link Inc | 501(c)(3) | 2026 | | | |
| Csi Gives Back Inc | 501(c)(3) | 2019 | | $1,837 | 2024-12 |
| Dakota S Love Foundation Inc | 501(c)(3) | 2024 | | | |
| Deborah Haynes Foundation Inc | 501(c)(3) | 2015 | $0 | $0 | 2025-12 |
| Discovery Health Place Inc | 501(c)(3) | 2023 | $0 | $0 | 2025-12 |
| Duval Car Club Cancer Awareness Inc | 501(c)(3) | 2019 | $0 | $0 | 2025-12 |
| Educating Minorities About Transplants Inc | 501(c)(3) | 2019 | $0 | $0 | 2025-12 |
| Elderly Transitional Services | 501(c)(3) | 2023 | $0 | $0 | 2024-12 |
| Equine Driven Empowerment Center Inc | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| First Coast Disaster Council Inc | 501(c)(3) | 2018 | $0 | $0 | 2025-12 |
| Florida Eye Foundation Inc | 501(c)(3) | 2019 | | $13,199 | 2025-12 |
| For Ashers Sake Foundation Inc | 501(c)(3) | 2015 | $0 | $0 | 2025-12 |
| Gateway Community Foundation Inc | 501(c)(3) | 2013 | $0 | $0 | 2025-06 |
| Gateway Health Systems Inc | 501(c)(3) | 2013 | $0 | $1 | 2025-06 |
| Gateway Transitional Services Inc | 501(c)(3) | 2013 | $0 | $0 | 2025-06 |
| Helpers Hand Foundation Incorporation | 501(c)(3) | 2023 | | | |
| Howellyeah Foundation | 501(c)(3) | 2026 | | | |
| Iahss North Florida Chapter | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| International Womens Obesity Network | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| Jessica Green Foundation Inc | 501(c)(3) | 2011 | $0 | $0 | 2025-12 |
| Jonathan Jean Foundation | 501(c)(3) | 2023 | $0 | $0 | 2025-12 |
| Joshua J Hillman Health Foundation Inc | 501(c)(3) | 2017 | $0 | $0 | 2025-12 |
| Justice League of Fitness- I Will Workout Inc | 501(c)(3) | 2016 | $0 | $0 | 2025-08 |
| Kaizen Health and Empowerment Found Ation Inc | 501(c)(3) | 2026 | | | |
| Latisha Deal Health Advocacy Firm | 501(c)(3) | 2025 | | | |
| Life Tree Women Care Services | 501(c)(3) | 2023 | | | |
| Little Heroes Medical Foundation in C | 501(c)(3) | 2026 | | | |
| Lone Warrior Foundation | 501(c)(3) | 2023 | $0 | $0 | 2024-12 |
| Mater Collective | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| North Central Florida Health Care Coalition Inc | 501(c)(3) | 2012 | $0 | $0 | 2025-12 |
| North East Florida Dental Hygiene Association | 501(c)(6) | 2019 | $0 | $0 | 2025-12 |
| North Florida Outreach and Rehabili Tation Services Inc | 501(c)(3) | 2026 | $0 | $0 | 2025-11 |
| Orsborn Foundation Inc | 501(c)(3) | 2018 | $0 | $0 | 2024-12 |
| Persown Care Inc | 501(c)(3) | 2025 | | | |
| Phoenix 4 Life Foundation | 501(c)(3) | 2024 | | | |
| Pickandchooselife Inc | 501(c)(3) | 2024 | | | |
| Pink & the Blue Breast and Prostate Cancer Foundation Inc | 501(c)(3) | 2025 | | | |
| Pink Ribbon Blue Skies Foundation Inc | 501(c)(3) | 2022 | $0 | $0 | 2025-12 |
| Prestige Workforce Inc | 501(c)(3) | 2017 | $0 | $0 | 2024-12 |
| Project World of Joy and Caring | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Salud Y Sonrisas Waorani Inc | 501(c)(3) | 2025 | | | |
| Senior Veterans Forgotten Inc | 501(c)(3) | 2015 | $0 | $0 | 2023-12 |
| Shalem Foundation Inc | 501(c)(3) | 2025 | | | |
| Shands Jacksonville Healthcare Inc | 501(c)(3) | 1985 | $0 | $1.7 million | 2025-06 |
| Showering Gods Love Inc | 501(c)(3) | 2022 | $0 | $0 | 2025-12 |
| Sk8hers | 501(c)(3) | 2022 | $0 | $0 | 2025-12 |
| St Ann Marie Inc | 501(c)(3) | 2024 | | | |
| Success Opportunity Achievement and Reward Incorporate | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| The Pluto Foundation Inc | 501(c)(3) | 2026 | | | |
| Tops FL 765 Orange Park | 501(c)(3) | 2016 | $0 | $0 | 2026-03 |
| Transitioning Into New Beginnings Outreach Services | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Volunteer Docs Inc | 501(c)(3) | 2017 | $0 | $0 | 2025-12 |
| Volunteer Nurses for Wellness Inc | 501(c)(3) | 2017 | $0 | $0 | 2025-12 |