| Nona Jones Ministries Inc | 501(c)(3) | 2025 | $861,689 | $332,696 | 2025-12 |
| Christian Study Center of Gainesville Inc | 501(c)(3) | 2001 | $815,669 | $1.6 million | 2025-06 |
| Global Missions 365 Inc | 501(c)(3) | 2019 | $569,598 | $476,487 | 2024-12 |
| New Creation Fellowship Church Inc | 501(c)(3) | 2011 | $296,268 | $324,343 | 2025-12 |
| Campus House of Christian Campus Ministry Inc | 501(c)(3) | 2001 | $276,616 | $1.3 million | 2025-12 |
| Impact for Living Inc | 501(c)(3) | 2006 | $275,985 | $356,692 | 2024-12 |
| Amazon Vision Ministries Inc | 501(c)(3) | 2005 | $245,572 | $488,050 | 2025-12 |
| Jewish Council of North Central Florida Inc | 501(c)(3) | 1996 | $231,289 | $307,143 | 2025-08 |
| House of Hope of Alachua County Inc | 501(c)(3) | 1997 | $167,395 | $411,605 | 2025-12 |
| Jim Gilbert Ministries Inc | 501(c)(3) | 1983 | $166,593 | $117,827 | 2024-12 |
| Krishna West Inc | 501(c)(3) | 2014 | $161,843 | $731 | 2025-12 |
| Beverly Crawford Ministries Inc | 501(c)(3) | 2016 | $141,058 | $340 | 2024-12 |
| Bob and Rose Weiner Ministries Inc | 501(c)(3) | 1991 | $122,494 | $150,041 | 2024-12 |
| 1010 Waqf Inc | 501(c)(3) | 2026 | $81,582 | $56,188 | 2024-12 |
| Miracle Life Ministries Inc | 501(c)(3) | 1991 | $74,899 | $44,788 | 2025-12 |
| Chinese Collegial Ministry Corp | 501(c)(3) | 2025 | $58,585 | $26,987 | 2025-12 |
| Master Builder Christian International University Inc | 501(c)(3) | 2023 | $45,119 | $33,507 | 2025-12 |
| Redeemed Christian Church of God Throne of Grace Tabernacle | 501(c)(3) | 2019 | $42,832 | $6,033 | 2018-12 |
| Mark Vega Evangelistic Ministries Inc | 501(c)(3) | 2015 | $22,577 | $16,495 | 2024-12 |
| A Time of Refreshing Prayer and Word Womens Ministry Inc | 501(c)(3) | 2019 | $0 | $0 | 2024-12 |
| Abundant Love International Ministries Inc | 501(c)(3) | 2003 | | | |
| Acacias Ark Incorporated | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Alive Church Inc | 501(c)(3) | 2007 | | | |
| All Saints Anglican Church Gainesville Inc | 501(c)(3) | 2001 | | | |
| Alpha Retreat Catholic Ministry Inc | 501(c)(3) | 2012 | $0 | $0 | 2025-12 |
| Amitabha Buddhist Association Inc | 501(c)(3) | 2020 | $0 | $0 | 2025-12 |
| Anointing of the Holy Spirit Ministry | 501(c)(3) | 2000 | | | |
| Antioch United Holiness Church | 501(c)(3) | 2009 | | | |
| Bondage Breaking Ministries Inc | 501(c)(3) | 2011 | | | |
| C1 Network Inc | 501(c)(3) | 2022 | $0 | $0 | 2025-12 |
| Casting the Net Ministries Inc | 501(c)(3) | 2011 | $0 | $0 | 2025-12 |
| Catalyst Gainesville Inc | 501(c)(3) | 2005 | | | |
| Champions Ministries Gainesville Inc | 501(c)(3) | 2003 | | | |
| Chapel of Gainesville Inc | 501(c)(3) | 2014 | | | |
| Charity Family Church | 501(c)(3) | 2014 | | | |
| Children of God Centers Inc | 501(c)(3) | 2001 | $0 | $0 | 2025-12 |
| Christ Harvest Ministries Inc | 501(c)(3) | 2017 | | | |
| Christian Pastors Association of North Central Florida Inc | 501(c)(3) | 2006 | | | |
| Church of the Kingdom of God Inc | 501(c)(3) | 2002 | | | |
| Church of the Living God Pillar and Ground of the Truth in Gainesvill | 501(c)(3) | 2018 | | | |
| Clarity Ministries in Unity | 501(c)(3) | 2006 | | | |
| Congregation Bnai Israel | 501(c)(3) | 2018 | | | |
| Dai Tong Lam Buddhist Association Inc | 501(c)(3) | 2009 | | | |
| Deliverance Gospel Temple Worship | 501(c)(3) | 2002 | | | |
| Emanuel Missionary Baptist Church Inc | 501(c)(3) | 2008 | | | |
| Esoteric Interfaith Church Inc | 501(c)(3) | 2007 | | | |
| Faith Christian Family Church Inc | 501(c)(3) | 2011 | $0 | $0 | 2025-12 |
| Faith Tabernacle Missionary Baptist Church | 501(c)(3) | 1969 | | | |
| Faith Tabernacle of Praise Ministries Inc | 501(c)(3) | 2005 | | | |
| Feed My Sheep Mission Inc | 501(c)(3) | 1998 | | | |
| Fire of God Ministries Inc | 501(c)(3) | 2006 | | | |
| First Interdenominational Christian Church Org Corp | 501(c)(3) | 2024 | | | |
| Fountain of Restoration Ministries | 501(c)(3) | 2007 | | | |
| Fresh Start Ministries of Gainesville | 501(c)(3) | 2020 | | | |
| Friends and Charities Development Inc | 501(c)(3) | 2009 | $0 | $0 | 2025-12 |
| Furnace House of Prayer Inc | 501(c)(3) | 2013 | | | |
| GNV Bridge Inc | 501(c)(3) | 2020 | $0 | $0 | 2025-12 |
| Gainesville Chinese Christian Church | 501(c)(3) | 2015 | | | |
| Gainesville Family Worship Center | 501(c)(3) | 2006 | | | |
| Gainesville International Christian Church Inc | 501(c)(3) | 2024 | | | |
| Gainesville Pinal Or Inc | 501(c)(3) | 2002 | | | |
| Gateway Christian Center Inc | 501(c)(3) | 2001 | | | |
| Goshen Christian Church | 501(c)(3) | 2017 | $0 | $0 | 2025-12 |
| Grace Encouragement Ministries | 501(c)(3) | 2004 | $0 | $0 | 2024-12 |
| Grace Temple Outreach Ministriesinc | 501(c)(3) | 2007 | | | |
| Grace at Fort Clarke Church Inc | 501(c)(3) | 2025 | | | 2013-12 |
| Grand View Church Inc | 501(c)(3) | 2014 | | | |
| Greater Love and Faith Ministries Inc | 501(c)(3) | 2012 | | | |
| HDG Global Publications Inc | 501(c)(3) | 2023 | $0 | $0 | 2025-12 |
| HDG Legacy Trust | 501(c)(3) | 2021 | $0 | $0 | 2025-10 |
| Higher Anointing Community Harvest Center Inc | 501(c)(3) | 2000 | | | |
| Holy Temple of God Incorporated | 501(c)(3) | 2002 | | | |
| Hope To the World Church | 501(c)(3) | 2006 | | | |
| House of Bountiful Mercy Ministry Inc | 501(c)(3) | 2002 | | | |
| House of Fire Ministries Inc | 501(c)(3) | 2007 | | | |
| Iglesia Faro de Salvacion Incorporated | 501(c)(3) | 2023 | | | |
| Iglesia Hispana Gainesville Inc | 501(c)(3) | 2024 | | | |
| Ignited Faith Ministries Inc | 501(c)(3) | 2021 | | | |
| Institute for Advanced Social Design Inc | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| Ipul Newberry Inc | 501(c)(3) | 2025 | | | |
| Iskcon Resolve Inc | 501(c)(3) | 2026 | | | |
| Jesus Christ of Nazareth Worship and Praise Temple Inc | 501(c)(3) | 2002 | | | |
| Jesus People Life Changing Ministry Church Inc | 501(c)(3) | 1998 | | | |
| Johnson Chapel Missionary Baptist Church Inc | 501(c)(3) | 2018 | $0 | $0 | 2025-12 |
| Joseph Singletary Ministries Inc | 501(c)(3) | 2006 | | | |
| Kingdom Life Ministries | 501(c)(3) | 2006 | | | |
| Kingdom Minded Ministries | 501(c)(3) | 2020 | | | |
| Korean Baptist Church | 501(c)(3) | 1969 | | | |
| Krishna House North America Inc | 501(c)(3) | 2024 | $0 | $0 | 2024-12 |
| Living Water Church of Gainesville | 501(c)(3) | 2024 | | | |
| Living Water Women S Ministry Inc | 501(c)(3) | 2019 | $0 | $0 | 2025-12 |
| Looking4answers Inc | 501(c)(3) | 2020 | $0 | $0 | 2025-12 |
| Lubavitch-Chabad Jewish Center of Gainesville | 501(c)(3) | 2001 | | | |
| Meizon Mission | 501(c)(3) | 2022 | | | |
| Mercy Corporation | 501(c)(3) | 2008 | $0 | $0 | 2025-12 |
| Ministerio Internacional Vino Nuevoinc | 501(c)(3) | 2008 | | | |
| Miracle Word of Faith Ministries Inc | 501(c)(3) | 2004 | | | |
| More Than Conquerors Faith and Outreach Center Inc | 501(c)(3) | 2005 | | | |
| Mount Carmel Baptist Church of Gainesville Inc | 501(c)(3) | 2016 | | | |
| Multiply Disciples Inc | 501(c)(3) | 2023 | $0 | $0 | 2025-12 |
| National Missionary Baptist House of Prayer Holy Ghost Outreach | 501(c)(3) | 1996 | | | |
| New Horizon Outreach Ministry Incorporated | 501(c)(3) | 2012 | | | |
| New Life Beginning Church Inc | 501(c)(3) | 2013 | | | |
| New Life Believers Outreach | 501(c)(3) | 2024 | $0 | $0 | 2025-12 |
| New Life Community Chapel of Gaines Ville Inc | 501(c)(3) | 2026 | | | |
| New World Outreach Ministries Inc | 501(c)(3) | 2019 | | | |
| North Central Florida Churches of Christ Christian Brothers Benevole | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| North Gainesville Baptist Church Inc | 501(c)(3) | 2017 | | | |
| Open Alliance for the Study of Islamic Spirituality Inc | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| Overcomers Through Christ Ministries Inc | 501(c)(3) | 2016 | | | |
| Passage Ministries | 501(c)(3) | 2002 | | | |
| Pentecostal Praise and Deliverance Church Inc | 501(c)(3) | 2014 | $0 | $0 | 2025-12 |
| Pineapple Principle Inc | 501(c)(3) | 2022 | $0 | $0 | 2024-12 |
| Powerhouse Family Worship Center Inc | 501(c)(3) | 2013 | | | |
| Praize Center Incorporated | 501(c)(3) | 2016 | | | |
| Prayer & Deliverance Ministry Incorporated | 501(c)(3) | 2016 | $0 | $0 | 2024-12 |
| Prevailing Word Bible Ministry Inc | 501(c)(3) | 2014 | | | |
| Promise Driven Church | 501(c)(3) | 2011 | | | |
| Ramah Praise Outreach Ministries Inc | 501(c)(3) | 2004 | | | |
| Redeemed Christian Church of Godgrace and Mercy Chapel | 501(c)(3) | 2019 | | | |
| Redeemed International Evangelistic Association | 501(c)(3) | 2001 | $0 | $0 | 2025-12 |
| Redeeming Faith and Anointing Ministries Inc | 501(c)(3) | 1998 | | | |
| Right Word Church Inc | 501(c)(3) | 2016 | | | |
| Ripple Missions | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| Rupa Raghunatha Gaudiya Matha | 501(c)(3) | 2003 | | | |
| Saint Andrews Church of Gainesville Inc | 501(c)(3) | 1995 | | | |
| Salt Church of Gainesville Inc | 501(c)(3) | 2019 | | | |
| Seraphim Center of Gainesville Inc | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| Shekinah Glory Family Worship Center | 501(c)(3) | 2016 | | | |
| Shift Church | 501(c)(3) | 2014 | | | |
| Shift Community Church Inc | 501(c)(3) | 2023 | | | |
| Sonlife Church of Christ Inc | 501(c)(3) | 2024 | | | |
| Southeast 10TH Avenue Church of Christ | 501(c)(3) | 2026 | | | |
| Southeastern Buddhist Retreat Center | 501(c)(3) | 2024 | $0 | $0 | 2024-12 |
| Southside Christian Charities of FL Orida Inc | 501(c)(3) | 2026 | $0 | $0 | 2025-12 |
| Spirit and Truth Catholic Ministry | 501(c)(3) | 2023 | $0 | $0 | 2025-12 |
| Spiritual Assembly of the Bahais of Alachua County | 501(c)(3) | 1995 | | | |
| Sridhar Puri Global Mission | 501(c)(3) | 2025 | | | |
| St Barbara CFC Ministries Inc | 501(c)(3) | 2019 | | | |
| St Elizabeth Greek Orthodox Church | 501(c)(3) | 2022 | | | |
| St Mary Magdalene Coptic Orthodox Church | 501(c)(3) | 2019 | | | |
| Starlight Temple House of | 501(c)(3) | 2009 | | | |
| Strong Tower Ministries | 501(c)(3) | 2002 | | | |
| Sumak Kawsay - Iglesia Del Buen Vivir Foundation Corporation | 501(c)(3) | 2024 | | | |
| Swords Into Plowshares Inc | 501(c)(3) | 2025 | $0 | $0 | 2025-12 |
| TL Williams Evangelistic Associatio N Inc | 501(c)(3) | 2026 | $0 | $0 | 2025-12 |
| The Fiat Center | 501(c)(3) | 2026 | | | |
| The Full Cornerstone Gospel Church Inc | 501(c)(3) | 2002 | | | |
| The Gainesville Church Inc | 501(c)(3) | 2002 | | | 2018-12 |
| The Word Church Global Inc | 501(c)(3) | 2011 | | | |
| Transformation Bible Vision Ministries Incorporated | 501(c)(3) | 2014 | | | |
| Tree City Church | 501(c)(3) | 2026 | | | |
| Trinity Christian Ministries Inc | 501(c)(3) | 1999 | | | |
| Upper Garden Sanctuary Inc | 501(c)(3) | 2026 | | | |
| Victorious Faith Inc | 501(c)(3) | 2019 | | | |
| Victorious Faith and Love Christian Fellowship Inc | 501(c)(3) | 2006 | | | |
| Victory Temple Ministries Church of God in Christ Incorporated | 501(c)(3) | 2006 | | | |
| Vida Springs Church Inc | 501(c)(3) | 2016 | | | |
| Vietnamese Buddhist Meditation Congregation Truc Lam Tue Tu | 501(c)(3) | 2024 | $0 | $0 | 2025-03 |
| Walk of Life Deliverance Ministries | 501(c)(3) | 2019 | | | |
| Watts Teaching Ministries Inc | 501(c)(3) | 2005 | $0 | $0 | 2025-12 |
| Williams Temple Church of God in Christ | 501(c)(3) | 2023 | | | |