Data / Federal rules / 2025-19119
Procedures for Transportation Workplace Drug and Alcohol Testing Programs
The Transportation Department published this proposed rule in the Federal Register on October 1, 2025. Comments closed on November 15, 2025.
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What the agency says
This action supplements an earlier notice of proposed rulemaking (NPRM) that DOT published on December 9, 2024. This supplemental proposal would update terminology in DOT's drug and alcohol testing regulations consistent with Executive Order 14168 (E.O. 14168), Defending Women from Gender Ideology Extremism and Restoring Biological Truth to the Federal Government. DOT continues to propose a provision to require a directly observed urine collection in situations where oral fluid tests are currently required, but oral fluid testing is not yet available.
Key facts
- Document number
- 2025-19119
- Type
- Proposed rule
- Action
- Supplemental notice of proposed rulemaking
- Agency
- Transportation Department
- Published
- October 1, 2025
- Comments due
- November 15, 2025
- Dates
- Comments on this notice of proposed rulemaking should be submitted by November 15, 2025.
- Docket
- Docket DOT-OST-2021-0093
- Regulation ID (RIN)
- 2105-AF28
- Code of Federal Regulations
- 49 CFR 40
- Topics
- Administrative practice and procedure; Alcohol abuse; Drug abuse; Drug testing; Laboratories; Reporting and recordkeeping requirements; Safety; Transportation
- Pages
- 5
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Everything from the Transportation Department · Rules on administrative practice and procedure · Rules on alcohol abuse · Rules on drug abuse · Published in October 2025
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